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Literature summary for 1.3.1.20 extracted from
- Site-directed mutagenesis studies of bovine liver cytosolic dihydrodiol dehydrogenase: the role of Asp-50, Tyr-55, Lys-84, His-117, Cys-145 and Cys-193 in enzymatic activity (2001), Chem. Biol. Interact., 130-132, 833-845.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged protein in Escherichia coli | Bos taurus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C145S | same sensitivity to SH-reagents as wild-type | Bos taurus |
| C193S | no sensitivity to SH-reagents remaining | Bos taurus |
| D50N | low dehydrogenase activity | Bos taurus |
| H117Q | increased reductase activity | Bos taurus |
| K84N | low dehydrogenase activity, no remaining reductase activity | Bos taurus |
| Y55F | very low dehydrogenase activity | Bos taurus |
| Y55H | decreased dehydrogenase activity, significant increased in reductase activity | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | i.e. DTNB, inhibits wild-type enzyme and mutant C145S | Bos taurus |  |
| glutathione | wild-type and mutant C145S | Bos taurus | |
| N-ethylmaleimide | wild-type and mutant C145S | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bos taurus | metabolic inactivation of exogeneous xenobiotics | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
isoform DD3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DD3, recombinant, His-tagged protein from Escherichia coli | Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH + H+ | structure analysis reveals that the enzyme belongs to the aldo-keto reductase superfamily, like 3alpha-hydroxysteroid dehydrogenase, 17beta-hydroxysteroid dehydrogenase, 20alpha-hydroxysteroid dehydrogenase, aldose reductase, and aldehyde reductase | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-acenaphthenol + NAD(P)+ | - |
Bos taurus | ? | - |
? | |
| camphorquinone + NAD(P)H | - |
Bos taurus | (1R)-1,7,7-trimethylbicyclo[2.2.1]heptane-2,3-diol + NADP+ | - |
? | |
| additional information | metabolic inactivation of exogeneous xenobiotics | Bos taurus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DD | - |
Bos taurus |
| dihydrodiol dehydrogenase | - |
Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
mutant H117Q of DD3 | Bos taurus |
| 9.7 | 10.5 | mutant K84N of DD3 | Bos taurus |
| 10 | - |
mutant D50N of DD3 | Bos taurus |
| 10.5 | - |
DD3, wild-type | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Bos taurus | |
| NADPH | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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