Literature summary for 1.3.1.25 extracted from

Harayama, S.; Rekik, M.
The meta cleavage operon of TOL degradative plasmid pWW0 comprises 13 genes (1990), Mol. Gen. Genet., 221, 113-120.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of xylL gene in Escherichia coli strain from TOL plasmid	Pseudomonas putida

Protein Variants

Protein Variants	Comment	Organism
additional information	operon insertion mutants of recombinant Escherichia coli expressing the TOL plasmid encoded enzymes including 1,2-dihydroxy--3,5-cyclohexadiene-1-carboxylate dehydrogenase, sequence analysis	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	? * 28000, DNA sequence determination, recombinant from Escherichia coli	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+	Pseudomonas putida	-	catechol + CO2 + NADH	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from Escherichia coli	Pseudomonas putida

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+	-	Pseudomonas putida	catechol + CO2 + NADH	-	?

Subunits

Subunits	Comment	Organism
?	? * 28000, DNA sequence determination, recombinant from Escherichia coli	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Pseudomonas putida

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Release 2023.1 (January 2023)