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Literature summary for 1.3.1.29 extracted from
- Purification and properties of (plus)-cis-naphthalene dihydrodiol dehydrogenase of Pseudomonas putida (1974), J. Bacteriol., 119, 879-888.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,2'-dipyridyl | 16.7 mM, 55% inhibition | Pseudomonas putida |  |
| 8-hydroxyquinoline | 16.7 mM, 25% inhibition | Pseudomonas putida | |
| EDTA | 16.7 mM, 17% inhibition | Pseudomonas putida | |
| p-chloromercuribenzoate | 16.7 mM, 87% inhibition | Pseudomonas putida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
NAD+ | - |
Pseudomonas putida | |
| 0.8 | - |
cis-1,2-dihydronaphthalene-1,2-diol | - |
Pseudomonas putida | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25500 | - |
4 * 25500, SDS-PAGE | Pseudomonas putida |
| 102000 | - |
gel filtration | Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
strains NP, PL6 and C84-15 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas putida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pseudomonas putida |
| 8.43 | - |
- |
Pseudomonas putida |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 11 days, little loss of activity | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ | i.e. cis-naphthalene dihydrodiol, specific for (+)-isomers | Pseudomonas putida | 1,2-dihydroxynaphthalene + NADH | - |
? | |
| cis-1,2-dihydronaphthalene-1,2-diol + NAD+ | - |
Pseudomonas putida | naphthalene-1,2-diol + NADH + H+ | - |
r | |
| cis-anthracene dihydrodiol + NAD+ | 56% of activity with cis-naphthalene dihydrodiol | Pseudomonas putida | 1,2-dihydroxyanthracene + NADH | - |
? | |
| cis-benzene dihydrodiol + NAD+ | 4% of activity with cis-naphthalene dihydrodiol | Pseudomonas putida | 1,2-dihydroxybenzene + NADH | - |
? | |
| cis-biphenyl dihydrodiol + NAD+ | 43% of activity with cis-naphthalene dihydrodiol | Pseudomonas putida | 3-phenyl-1,2-dihydroxybenzene + NADH | - |
? | |
| cis-ethylbenzene dihydrodiol + NAD+ | 48% of activity with cis-naphthalene dihydrodiol | Pseudomonas putida | 3-ethyl-1,2-dihydroxybenzene + NADH | - |
? | |
| cis-phenanthrene dihydrodiol + NAD+ | 53% of activity with cis-naphtalene dihydrodiol | Pseudomonas putida | 3,4-dihydroxyphenanthrene + NADH | - |
? | |
| cis-toluene dihydrodiol + NAD+ | 26% of activity with cis-naphthalene dihydrodiol | Pseudomonas putida | 3-methyl-1,2-dihydroxybenzene + NADH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 25500, SDS-PAGE | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | 9 | - |
Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | absolutely required | Pseudomonas putida | |
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