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Literature summary for 1.3.1.3 extracted from
- Identification of cortisone 5beta-reductase as DELTA4-3-ketosteroid 5beta-reductase (1987), Biochim. Biophys. Acta, 912, 110-114.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | - |
Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
cortisone | - |
Rattus norvegicus | |
| 0.0022 | - |
4-androstene-3,17-dione | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-androstene-3,17-dione + NADPH | Rattus norvegicus | - |
5beta-androstan-3,17-dione + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein | - |
| Rattus norvegicus | - |
cortisone 5beta-reductase and reduction of androstenedione (3-oxo-5beta-steroid:NADP+ DELTA4-oxidoreductase activity, EC 1.3.1.23) catalyzed by the same catalytic site of a single protein | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
| cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.034 | - |
purified enzyme, substrate cortisone | Rattus norvegicus |
| 0.0341 | - |
- |
Rattus norvegicus |
| 0.0354 | - |
purified enzyme, substrate androstenedione | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-androstene-3,17-dione + NADPH | - |
Rattus norvegicus | 5beta-androstan-3,17-dione + NADP+ | - |
? | |
| androst-4-en-3,17-dione + NADPH | - |
Rattus norvegicus | 5beta-androstan-3,17-dione + NADP+ | - |
? | |
| cortisone + NADPH + H+ | - |
Rattus norvegicus | 5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
60 s, 50% loss of activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Rattus norvegicus | |
| NADPH | absolutely required | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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