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Literature summary for 1.3.1.31 extracted from
- Additional enoates amd other alpha,beta-unsaturated carbonyl compounds as substrates for the enoate reductase from Clostridium tyrobutyricum, influence of elevated hydrogen pressure on the reduction rate (1989), DECHEMA Biotechnol. Conf., 3, 189-192.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
12-phenyldodec-2-enoic acid | - |
Clostridium tyrobutyricum |  |
| 0.029 | - |
(2E)-2-methyl-3-phenylacrylic acid | - |
Clostridium tyrobutyricum | |
| 0.037 | - |
(2E)-2-methyl-3-phenylbut-2-enoic acid | - |
Clostridium tyrobutyricum | |
| 0.15 | - |
(2E)-3-phenylacrylic acid | - |
Clostridium tyrobutyricum | |
| 1.4 | - |
oct-2-en-4-ynoic acid | - |
Clostridium tyrobutyricum | |
| 1.7 | - |
4-methoxy-2-methyl-4-oxobutanoic acid | - |
Clostridium tyrobutyricum | |
| 7.4 | - |
(2E)-2-methylbut-2-enedioic acid | - |
Clostridium tyrobutyricum | |
| 16.2 | - |
4-methoxy-3-methyl-4-oxobutanoic acid | - |
Clostridium tyrobutyricum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium tyrobutyricum | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E)-2-methyl-3-phenylacrylic acid + electron donor | - |
Clostridium tyrobutyricum | 2-methyl-3-phenylpropanoic acid + electron acceptor | - |
? | |
| (2E)-2-methyl-3-phenylbut-2-enoic acid + electron donor | - |
Clostridium tyrobutyricum | 2-methyl-3-phenylbutanoic acid + electron acceptor | - |
? | |
| (2E)-2-methylbut-2-enedioic acid + electron donor | - |
Clostridium tyrobutyricum | 2-methylbutanedioic aicd + electron acceptor | - |
? | |
| (2E)-3-phenylacrylic acid + electron donor | - |
Clostridium tyrobutyricum | 3-phenylpropanoic acid + electron acceptor | - |
? | |
| (2E)-4-methoxy-2-methyl-4-oxobut-2-enoic acid + electron donor | - |
Clostridium tyrobutyricum | 4-methoxy-2-methyl-4-oxobutanoic acid + electron acceptor | - |
? | |
| (2E)-4-methoxy-3-methyl-4-oxobut-2-enoic acid + electron donor | - |
Clostridium tyrobutyricum | 4-methoxy-3-methyl-4-oxobutanoic acid + electron acceptor | - |
? | |
| (2E,4E)-2,3,5,8-tetramethylnona-2,4,7-trienoic acid + electron donor | - |
Clostridium tyrobutyricum | ? + electron acceptor | - |
? | |
| (2E,4E)-2,3-dimethylhexa-2,4-dienoic acid + electron donor | - |
Clostridium tyrobutyricum | ? + electron acceptor | - |
? | |
| (E)-2-oxo-4-phenyl-3-butenoate + NADH | 18% of the activity with butenoate | Clostridium tyrobutyricum | 2-oxo-4-phenyl-3-butanoate + NAD+ | - |
? | |
| (E)-3-methyl-2-oxo-4-phenyl-3-butenoate + NADH | 27% of the activity with butenoate | Clostridium tyrobutyricum | 3-methyl-2-oxo-4-phenyl-3-butenoate + NAD+ | - |
? | |
| 12-phenyldodec-2-enoic acid + electron donor | - |
Clostridium tyrobutyricum | 12-phenyldodecanoic acid + electron acceptor | - |
? | |
| butenoate + NADH | - |
Clostridium tyrobutyricum | butanoate + NAD+ | - |
? | |
| hexa-2,4-dienoic acid + electron donor | - |
Clostridium tyrobutyricum | ? + electron acceptor | - |
? | |
| oct-2-en-4-ynoic acid + electron donor | - |
Clostridium tyrobutyricum | ? + electron acceptor | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains 1 mol per mol of subunit | Clostridium tyrobutyricum | |
| FMN | contains 1 mol per mol of subunit | Clostridium tyrobutyricum | |
| NADH | - |
Clostridium tyrobutyricum | |
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