Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mersalyl | 1 mM, complete inhibition | Clostridium sp. | |
O2 | in the reduced state the enzyme is inactivated in 1-2 min, about 3 h after the removal of oxygen, the activity is partially restored | Clostridium sp. | |
p-hydroxymercuribenzoate | 0.1 mM, complete inhibition | Clostridium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Clostridium kluyveri | |
additional information | - |
additional information | Km-value for NADH is lower than 0.02 mM | Clostridium sp. | |
0.02 | - |
cinnamate | - |
Clostridium sp. | |
0.8 | - |
(E)-2-butenoate | - |
Clostridium sp. | |
1.67 | - |
(E)-2-methyl-2-butenoate | - |
Clostridium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | iron-sulfur protein, contains 3.5-3.8 atoms of iron and 4.0 atoms of labile sulfur per subunit | Clostridium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
6 * 78000, SDS-PAGE | Clostridium sp. |
450000 | - |
gel filtration | Clostridium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium kluyveri | - |
- |
- |
Clostridium sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Clostridium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.9 | - |
- |
Clostridium sp. |
Storage Stability | Organism |
---|---|
-20°C, phosphate buffer, pH 7.0, containing (E)-2-methyl-2-butenoate, EDTA, sucrose and 2-mercaptoethanol, repeatedly thawed and frozen, 50% loss of activity | Clostridium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-2-butenoate + NADH | - |
Clostridium kluyveri | butanoate + NAD+ | - |
? | |
(E)-2-butenoate + NADH | - |
Clostridium sp. | butanoate + NAD+ | - |
? | |
(E)-2-methyl-2-butenoate + NADH | - |
Clostridium kluyveri | 2-methylbutanoate + NAD+ | - |
? | |
(E)-2-methyl-2-butenoate + NADH | - |
Clostridium sp. | 2-methylbutanoate + NAD+ | - |
? | |
(E)-3-methylpentenoate + NADH | - |
Clostridium kluyveri | 3-methylpentanoate + NAD+ | - |
? | |
(E)-3-methylpentenoate + NADH | - |
Clostridium sp. | 3-methylpentanoate + NAD+ | - |
? | |
(E)-cinnamate + NADH | - |
Clostridium kluyveri | phenylpropionate + NAD+ | - |
? | |
(E)-cinnamate + NADH | - |
Clostridium sp. | phenylpropionate + NAD+ | - |
? | |
(Z)-3-methylpentenoate + NADH | - |
Clostridium kluyveri | 3-methylpentanoate + NAD+ | - |
? | |
(Z)-3-methylpentenoate + NADH | - |
Clostridium sp. | 3-methylpentanoate + NAD+ | - |
? | |
butenoate + NADH | - |
Clostridium kluyveri | butanoate + NAD+ | - |
? | |
butenoate + NADH | reverse reaction is energetically extremely unfavorable | Clostridium sp. | butanoate + NAD+ | - |
? | |
cinnamate + NADH | - |
Clostridium sp. | phenylpropionate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 78000, SDS-PAGE | Clostridium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
20 min, stable | Clostridium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Clostridium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | enzyme contains 1 mol of FAD per mol of subunit | Clostridium sp. | |
NADH | - |
Clostridium kluyveri | |
NADH | - |
Clostridium sp. |