Cloned (Comment) | Organism |
---|---|
- |
Clostridium tyrobutyricum |
expressed in Escherichia coli growing under anaerobic conditions | Moorella thermoacetica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
x * 73000, SDS-PAGE | Moorella thermoacetica |
600000 | - |
gel filtration | Moorella thermoacetica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium tyrobutyricum | O52933 | - |
- |
Moorella thermoacetica | O52935 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Moorella thermoacetica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
- |
Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-2-methylbutenoate + NADH | - |
Clostridium tyrobutyricum | (R)-2-methylbutanoate + NAD+ | - |
? | |
(E)-2-methylbutenoate + NADH | - |
Moorella thermoacetica | (R)-2-methylbutanoate + NAD+ | - |
? | |
butenoate + NADH | - |
Clostridium tyrobutyricum | butanoate + NAD+ | - |
? | |
butenoate + NADH | - |
Moorella thermoacetica | butanoate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 73000, SDS-PAGE | Moorella thermoacetica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Moorella thermoacetica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | active between pH 5.0 and pH 9.0 | Moorella thermoacetica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Clostridium tyrobutyricum | |
NADH | - |
Moorella thermoacetica |