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Literature summary for 1.3.1.31 extracted from

  • Chaparro-Riggers, J.F.; Rogers, T.A.; Vazquez-Figueroa, E.; Polizzi, K.M.; Bommarius, A.S.
    Comparison of three enoate reductases and their potential use for biotransformations (2007), Adv. Synth. Catal., 349, 1521-1531.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Yersinia bercovieri
expressed in Escherichia coli BL21(DE3) Kluyveromyces lactis
expressed in Escherichia coli BL21(DE3) Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
2-cyclohexen-1-one the addition of 260 mM 2-cyclohexen-1-one leads to a more than 30fold decrease of the half-life at 30°C Yersinia bercovieri
additional information not inhibited by 2-cyclohexanone Yersinia bercovieri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.24
-
NADPH at 25°C and pH 7.5 Yersinia bercovieri

Organism

Organism UniProt Comment Textmining
Kluyveromyces lactis P40952 strain ATCC 8585D-5
-
Pseudomonas putida Q9R9V9 xenobiotic reductase A showing enoate reductase activity
-
Yersinia bercovieri
-
strain ATCC 43970
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA bead chromatography Yersinia bercovieri
Ni2+-NTA bead chromatography Kluyveromyces lactis
Ni2+-NTA bead chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.46
-
highest specific activity with 10 mM N-ethylmaleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C Kluyveromyces lactis
15.81
-
highest specific activity with 10 mM maleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C Yersinia bercovieri
25.82
-
highest specific activity with 10 mM maleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-carvone + NADPH
-
Yersinia bercovieri 2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+
-
?
(R)-carvone + NADPH
-
Kluyveromyces lactis 2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+
-
?
(R)-carvone + NADPH
-
Pseudomonas putida 2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+
-
?
2-cyclohexen-1-one + NADH
-
Yersinia bercovieri 2-cyclohexanone + NAD+
-
?
2-cyclohexen-1-one + NADH
-
Kluyveromyces lactis 2-cyclohexanone + NAD+
-
?
2-cyclohexen-1-one + NADH
-
Pseudomonas putida 2-cyclohexanone + NAD+
-
?
3-methyl-2-butenal + NADPH
-
Yersinia bercovieri 3-methylbutanal + NADP+
-
?
3-methyl-2-butenal + NADPH
-
Kluyveromyces lactis 3-methylbutanal + NADP+
-
?
3-methyl-2-butenal + NADPH
-
Pseudomonas putida 3-methylbutanal + NADP+
-
?
but-3-en-2-one + NADPH
-
Yersinia bercovieri butan-2-one + NADP+
-
?
but-3-en-2-one + NADPH
-
Kluyveromyces lactis butan-2-one + NADP+
-
?
but-3-en-2-one + NADPH
-
Pseudomonas putida butan-2-one + NADP+
-
?
cinnamaldehyde + NADPH
-
Yersinia bercovieri ?
-
?
cinnamaldehyde + NADPH
-
Kluyveromyces lactis ?
-
?
cinnamaldehyde + NADPH
-
Pseudomonas putida ?
-
?
citral + NADPH
-
Yersinia bercovieri 3-phenylpropanal + NADP+
-
?
citral + NADPH
-
Kluyveromyces lactis 3-phenylpropanal + NADP+
-
?
citral + NADPH
-
Pseudomonas putida 3-phenylpropanal + NADP+
-
?
ethyl 3-(tetrahydrofuran)propanoate + NADP+
-
Yersinia bercovieri ? + NADPH
-
?
ethyl 3-(tetrahydrofuran)propanoate + NADPH
-
Kluyveromyces lactis ? + NADP+
-
?
ethyl 3-(tetrahydrofuran)propanoate + NADPH
-
Pseudomonas putida ? + NADP+
-
?
ketoisophorone + NADPH
-
Yersinia bercovieri (6R)-levodione
-
?
ketoisophorone + NADPH
-
Kluyveromyces lactis (6R)-levodione
-
?
ketoisophorone + NADPH
-
Pseudomonas putida (6R)-levodione
-
?
maleic anhydride + NADPH
-
Yersinia bercovieri dihydrofuran-2,5-dione + NADP+
-
?
maleic anhydride + NADPH
-
Kluyveromyces lactis dihydrofuran-2,5-dione + NADP+
-
?
maleic anhydride + NADPH
-
Pseudomonas putida dihydrofuran-2,5-dione + NADP+
-
?
maleimide + NADH + H+
-
Yersinia bercovieri pyrrolidine-2,5-dione + NAD+
-
?
maleimide + NADH + H+
-
Kluyveromyces lactis pyrrolidine-2,5-dione + NAD+
-
?
maleimide + NADH + H+
-
Pseudomonas putida pyrrolidine-2,5-dione + NAD+
-
?
additional information NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide Yersinia bercovieri ?
-
?
additional information NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide Kluyveromyces lactis ?
-
?
additional information NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide Pseudomonas putida ?
-
?
N-ethylmaleimide + NADPH
-
Yersinia bercovieri N-ethylpyrrolidine-2,5-dione + NADP+
-
?
N-ethylmaleimide + NADPH
-
Kluyveromyces lactis N-ethylpyrrolidine-2,5-dione + NADP+
-
?
N-ethylmaleimide + NADPH
-
Pseudomonas putida N-ethylpyrrolidine-2,5-dione + NADP+
-
?
trans,trans-2,4-hexadienal + NADPH
-
Yersinia bercovieri ?
-
?
trans,trans-2,4-hexadienal + NADPH
-
Kluyveromyces lactis ?
-
?
trans,trans-2,4-hexadienal + NADPH
-
Pseudomonas putida ?
-
?

Synonyms

Synonyms Comment Organism
enoate reductase
-
Yersinia bercovieri
enoate reductase
-
Kluyveromyces lactis
enoate reductase
-
Pseudomonas putida
KYE1
-
Kluyveromyces lactis
NADPH dehydrogenase 1
-
Kluyveromyces lactis
old yellow enzyme
-
Yersinia bercovieri
old yellow enzyme
-
Kluyveromyces lactis
old yellow enzyme
-
Pseudomonas putida
XenA xenobiotic reductase A showing enoate reductase activity toward 2-cyclohexen-1-one Pseudomonas putida
Yers-ER
-
Yersinia bercovieri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 45 KYE1 has a half-life of 1146 min at 30°C, 210 min at 37°C, and less than 5 min at 45°C Kluyveromyces lactis
30 45 XenA has a half-life of 600 min at 30°C, 99 min at 37°C, and less than 5 min at 45°C Pseudomonas putida
30 45 Yers-ER has a half-life of more than 2160 min at 30°C, 1488 min at 37°C, and 117 min at 45°C Yersinia bercovieri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.97
-
NADPH at 25°C and pH 7.5 Yersinia bercovieri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Yersinia bercovieri

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5
-
Yersinia bercovieri

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9.5 the activity drops off sharply between pH 5-6 and pH 8.5-9.5 Yersinia bercovieri

Cofactor

Cofactor Comment Organism Structure
NADH the enzymes shows very little or no activity with NADH Yersinia bercovieri
NADH the enzymes shows very little or no activity with NADH Kluyveromyces lactis
NADH the enzymes shows very little or no activity with NADH Pseudomonas putida
NADPH preferred cofactor Yersinia bercovieri
NADPH preferred cofactor Kluyveromyces lactis
NADPH preferred cofactor Pseudomonas putida