Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Yersinia bercovieri |
expressed in Escherichia coli BL21(DE3) | Kluyveromyces lactis |
expressed in Escherichia coli BL21(DE3) | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-cyclohexen-1-one | the addition of 260 mM 2-cyclohexen-1-one leads to a more than 30fold decrease of the half-life at 30°C | Yersinia bercovieri | |
additional information | not inhibited by 2-cyclohexanone | Yersinia bercovieri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.24 | - |
NADPH | at 25°C and pH 7.5 | Yersinia bercovieri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces lactis | P40952 | strain ATCC 8585D-5 | - |
Pseudomonas putida | Q9R9V9 | xenobiotic reductase A showing enoate reductase activity | - |
Yersinia bercovieri | - |
strain ATCC 43970 | - |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA bead chromatography | Yersinia bercovieri |
Ni2+-NTA bead chromatography | Kluyveromyces lactis |
Ni2+-NTA bead chromatography | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.46 | - |
highest specific activity with 10 mM N-ethylmaleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C | Kluyveromyces lactis |
15.81 | - |
highest specific activity with 10 mM maleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C | Yersinia bercovieri |
25.82 | - |
highest specific activity with 10 mM maleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-carvone + NADPH | - |
Yersinia bercovieri | 2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+ | - |
? | |
(R)-carvone + NADPH | - |
Kluyveromyces lactis | 2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+ | - |
? | |
(R)-carvone + NADPH | - |
Pseudomonas putida | 2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+ | - |
? | |
2-cyclohexen-1-one + NADH | - |
Yersinia bercovieri | 2-cyclohexanone + NAD+ | - |
? | |
2-cyclohexen-1-one + NADH | - |
Kluyveromyces lactis | 2-cyclohexanone + NAD+ | - |
? | |
2-cyclohexen-1-one + NADH | - |
Pseudomonas putida | 2-cyclohexanone + NAD+ | - |
? | |
3-methyl-2-butenal + NADPH | - |
Yersinia bercovieri | 3-methylbutanal + NADP+ | - |
? | |
3-methyl-2-butenal + NADPH | - |
Kluyveromyces lactis | 3-methylbutanal + NADP+ | - |
? | |
3-methyl-2-butenal + NADPH | - |
Pseudomonas putida | 3-methylbutanal + NADP+ | - |
? | |
but-3-en-2-one + NADPH | - |
Yersinia bercovieri | butan-2-one + NADP+ | - |
? | |
but-3-en-2-one + NADPH | - |
Kluyveromyces lactis | butan-2-one + NADP+ | - |
? | |
but-3-en-2-one + NADPH | - |
Pseudomonas putida | butan-2-one + NADP+ | - |
? | |
cinnamaldehyde + NADPH | - |
Yersinia bercovieri | ? | - |
? | |
cinnamaldehyde + NADPH | - |
Kluyveromyces lactis | ? | - |
? | |
cinnamaldehyde + NADPH | - |
Pseudomonas putida | ? | - |
? | |
citral + NADPH | - |
Yersinia bercovieri | 3-phenylpropanal + NADP+ | - |
? | |
citral + NADPH | - |
Kluyveromyces lactis | 3-phenylpropanal + NADP+ | - |
? | |
citral + NADPH | - |
Pseudomonas putida | 3-phenylpropanal + NADP+ | - |
? | |
ethyl 3-(tetrahydrofuran)propanoate + NADP+ | - |
Yersinia bercovieri | ? + NADPH | - |
? | |
ethyl 3-(tetrahydrofuran)propanoate + NADPH | - |
Kluyveromyces lactis | ? + NADP+ | - |
? | |
ethyl 3-(tetrahydrofuran)propanoate + NADPH | - |
Pseudomonas putida | ? + NADP+ | - |
? | |
ketoisophorone + NADPH | - |
Yersinia bercovieri | (6R)-levodione | - |
? | |
ketoisophorone + NADPH | - |
Kluyveromyces lactis | (6R)-levodione | - |
? | |
ketoisophorone + NADPH | - |
Pseudomonas putida | (6R)-levodione | - |
? | |
maleic anhydride + NADPH | - |
Yersinia bercovieri | dihydrofuran-2,5-dione + NADP+ | - |
? | |
maleic anhydride + NADPH | - |
Kluyveromyces lactis | dihydrofuran-2,5-dione + NADP+ | - |
? | |
maleic anhydride + NADPH | - |
Pseudomonas putida | dihydrofuran-2,5-dione + NADP+ | - |
? | |
maleimide + NADH + H+ | - |
Yersinia bercovieri | pyrrolidine-2,5-dione + NAD+ | - |
? | |
maleimide + NADH + H+ | - |
Kluyveromyces lactis | pyrrolidine-2,5-dione + NAD+ | - |
? | |
maleimide + NADH + H+ | - |
Pseudomonas putida | pyrrolidine-2,5-dione + NAD+ | - |
? | |
additional information | NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide | Yersinia bercovieri | ? | - |
? | |
additional information | NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide | Kluyveromyces lactis | ? | - |
? | |
additional information | NADPH does not react at all in the presence of 2-cyclohexen-1-one and rarely in presence of maleimide | Pseudomonas putida | ? | - |
? | |
N-ethylmaleimide + NADPH | - |
Yersinia bercovieri | N-ethylpyrrolidine-2,5-dione + NADP+ | - |
? | |
N-ethylmaleimide + NADPH | - |
Kluyveromyces lactis | N-ethylpyrrolidine-2,5-dione + NADP+ | - |
? | |
N-ethylmaleimide + NADPH | - |
Pseudomonas putida | N-ethylpyrrolidine-2,5-dione + NADP+ | - |
? | |
trans,trans-2,4-hexadienal + NADPH | - |
Yersinia bercovieri | ? | - |
? | |
trans,trans-2,4-hexadienal + NADPH | - |
Kluyveromyces lactis | ? | - |
? | |
trans,trans-2,4-hexadienal + NADPH | - |
Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enoate reductase | - |
Yersinia bercovieri |
enoate reductase | - |
Kluyveromyces lactis |
enoate reductase | - |
Pseudomonas putida |
KYE1 | - |
Kluyveromyces lactis |
NADPH dehydrogenase 1 | - |
Kluyveromyces lactis |
old yellow enzyme | - |
Yersinia bercovieri |
old yellow enzyme | - |
Kluyveromyces lactis |
old yellow enzyme | - |
Pseudomonas putida |
XenA | xenobiotic reductase A showing enoate reductase activity toward 2-cyclohexen-1-one | Pseudomonas putida |
Yers-ER | - |
Yersinia bercovieri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | KYE1 has a half-life of 1146 min at 30°C, 210 min at 37°C, and less than 5 min at 45°C | Kluyveromyces lactis |
30 | 45 | XenA has a half-life of 600 min at 30°C, 99 min at 37°C, and less than 5 min at 45°C | Pseudomonas putida |
30 | 45 | Yers-ER has a half-life of more than 2160 min at 30°C, 1488 min at 37°C, and 117 min at 45°C | Yersinia bercovieri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.97 | - |
NADPH | at 25°C and pH 7.5 | Yersinia bercovieri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Yersinia bercovieri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | - |
Yersinia bercovieri |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | the activity drops off sharply between pH 5-6 and pH 8.5-9.5 | Yersinia bercovieri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the enzymes shows very little or no activity with NADH | Yersinia bercovieri | |
NADH | the enzymes shows very little or no activity with NADH | Kluyveromyces lactis | |
NADH | the enzymes shows very little or no activity with NADH | Pseudomonas putida | |
NADPH | preferred cofactor | Yersinia bercovieri | |
NADPH | preferred cofactor | Kluyveromyces lactis | |
NADPH | preferred cofactor | Pseudomonas putida |