Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Achromobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
2-methyl-N-phenylmaleimide | pH 7.5, 30°C | Achromobacter sp. | |
0.0191 | - |
NADH | pH 7.5, 30°C | Achromobacter sp. | |
0.0854 | - |
NADPH | pH 7.5, 30°C | Achromobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40600 | - |
calculated from cDNA | Achromobacter sp. |
45000 | - |
SDS-PAGE | Achromobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achromobacter sp. | I3V5V6 | - |
- |
Achromobacter sp. JA81 | I3V5V6 | - |
- |
Purification (Comment) | Organism |
---|---|
using affinity chromatography | Achromobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-methyl-N-phenylmaleimide + NADH + H+ | - |
Achromobacter sp. | (3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+ | - |
? | |
2-methyl-N-phenylmaleimide + NADH + H+ | - |
Achromobacter sp. JA81 | (3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+ | - |
? | |
2-methyl-N-phenylmaleimide + NADPH | - |
Achromobacter sp. | (3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+ | - |
? | |
2-methyl-N-phenylmaleimide + NADPH | - |
Achromobacter sp. JA81 | (3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Achr-OYE4 | - |
Achromobacter sp. |
enoate reductase | - |
Achromobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
optimal temperature in Tris-HCl buffer | Achromobacter sp. |
35 | - |
optimal temperature in potassium phosphate buffer | Achromobacter sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
about 40% of maximal activity maintained | Achromobacter sp. |
45 | - |
about 40% of maximal activity maintained | Achromobacter sp. |
55 | - |
no activity beyond 55°C | Achromobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
half-life of inactivation: 64.8 h | Achromobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
2-methyl-N-phenylmaleimide | pH 7.5, 30°C | Achromobacter sp. | |
19.2 | - |
NADPH | pH 7.5, 30°C | Achromobacter sp. | |
38.6 | - |
NADH | pH 7.5, 30°C | Achromobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
highest activity using Tris-HCl | Achromobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
no activity at pH 11 | Achromobacter sp. |
9 | - |
only 30% of highest activity at pH 9 | Achromobacter sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Achromobacter sp. | |
NADH | preferred cofactor | Achromobacter sp. | |
NADPH | - |
Achromobacter sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
2-methyl-N-phenylmaleimide | pH 7.5, 30°C | Achromobacter sp. | |
220 | - |
NADPH | pH 7.5, 30°C | Achromobacter sp. | |
2000 | - |
NADH | pH 7.5, 30°C | Achromobacter sp. |