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Literature summary for 1.3.1.31 extracted from

  • Iqbal, N.; Rudroff, F.; Brige, A.; Van Beeumen, J.; Mihovilovic, M.D.
    Asymmetric bioreduction of activated carbon-carbon double bonds using Shewanella yellow enzyme (SYE-4) as novel enoate reductase (2012), Tetrahedron, 68, 7619-7623.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Shewanella sp.

Organism

Organism UniProt Comment Textmining
Shewanella sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-carvone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 100%, ee: 97% Shewanella sp. ?
-
?
(-)-carvone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 100%, ee: 95% Shewanella sp. ?
-
?
(2E)-2-benzylidenecyclohexanone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 92%, ee: 51% Shewanella sp. ?
-
?
(2E)-2-benzylidenecyclopentanone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 4%, ee: racemate Shewanella sp. ?
-
?
1,3-dimethyl-1H-pyrrole-2,5-dione + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99% Shewanella sp. ?
-
?
1-(cyclohex-1-en-1-yl)ethanone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 71%, ee: not applicable Shewanella sp. ?
-
?
1-(cyclopent-1-en-1-yl)ethanone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 30%, ee: not applicable Shewanella sp. ?
-
?
1-(prop-2-en-1-yl)-1H-pyrrole-2,5-dione + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: not applicable Shewanella sp. ?
-
?
1-butyl-3-methyl-1H-pyrrole-2,5-dione + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99% Shewanella sp. ?
-
?
1-ethyl-3-methyl-1H-pyrrole-2,5-dione + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99% Shewanella sp. ?
-
?
2-methylcyclopent-2-enone + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 92%, ee: 52% Shewanella sp. ?
-
?
3-methyl-1-(prop-2-en-1-yl)-1H-pyrrole-2,5-dione + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99% Shewanella sp. ?
-
?
3-methyl-1-propyl-1H-pyrrole-2,5-dione + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99% Shewanella sp. ?
-
?
diethyl (2Z)-2-methylbut-2-enedioate + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99% Shewanella sp. ?
-
?
dimethyl (2Z)-2-methylbut-2-enedioate + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 98% Shewanella sp. ?
-
?
dimethyl 2-methylidenebutanedioate + NADPH biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 19%, ee: 90% Shewanella sp. ?
-
?

Synonyms

Synonyms Comment Organism
enoate reductase
-
Shewanella sp.
SYE-4
-
Shewanella sp.
yellow enzyme
-
Shewanella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Shewanella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Shewanella sp.