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Literature summary for 1.3.1.32 extracted from

  • Mueller, D.; Schloemann, M.; Reineke, W.
    Maleylacetate reductases in chloroaromatic-degrading bacteria using the modified ortho pathway: comparison of catalytic properties (1996), J. Bacteriol., 178, 298-300.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
5-fluoromaleylacetate
-
Cupriavidus necator
0.022
-
5-fluoromaleylacetate
-
Pseudomonas aeruginosa
0.029
-
3-chloromaleylacetate
-
Pseudomonas aeruginosa
0.03
-
3-chloromaleylacetate
-
Cupriavidus necator
0.044
-
5-chloromaleylacetate
-
Cupriavidus necator
0.05
-
maleylacetate
-
Cupriavidus necator
0.05
-
5-chloromaleylacetate
-
Pseudomonas aeruginosa
0.052
-
2-chloromaleylacetate
-
Cupriavidus necator
0.063
-
3-bromomaleylacetate
-
Cupriavidus necator
0.065
-
maleylacetate
-
Pseudomonas aeruginosa
0.071
-
3-bromomaleylacetate
-
Pseudomonas aeruginosa
0.078
-
2-chloromaleylacetate
-
Pseudomonas aeruginosa
0.084
-
5-bromomaleylacetate
-
Cupriavidus necator
0.095
-
5-bromomaleylacetate
-
Pseudomonas aeruginosa
0.098
-
2-bromomaleylacetate
-
Cupriavidus necator
0.117
-
2-bromomaleylacetate
-
Pseudomonas aeruginosa
0.125
-
5-methylmaleylacetate
-
Pseudomonas aeruginosa
0.133
-
5-methylmaleylacetate
-
Cupriavidus necator
0.17
-
2,3-dichloromaleylacetate
-
Pseudomonas aeruginosa
0.288
-
2,5-dichloromaleylacetate
-
Pseudomonas aeruginosa
0.292
-
3-methylmaleylacetate
-
Cupriavidus necator
0.33
-
2-methylmaleylacetate
-
Cupriavidus necator
0.365
-
3-methylmaleylacetate
-
Pseudomonas aeruginosa
0.374
-
2-methylmaleylacetate
-
Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
2 * 37000, SDS-PAGE Pseudomonas aeruginosa
74000
-
-
Pseudomonas aeruginosa
74000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-maleylacetate + NADPH Pseudomonas aeruginosa
-
3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH Pseudomonas sp.
-
3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH Cupriavidus necator
-
3-oxoadipate + NADP+
-
ir

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
JMP 134
-
Cupriavidus necator JMP 134-1
-
JMP 134
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas aeruginosa RHO1
-
-
-
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa
-
Cupriavidus necator

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
170
-
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3,5-trichloromaleylacetate + NADH
-
Pseudomonas aeruginosa 2,4-dichloro-3-oxohexanedioic acid + NAD+ + Cl-
-
?
2,3,5-trichloromaleylacetate + NADH
-
Pseudomonas sp. 2,4-dichloro-3-oxohexanedioic acid + NAD+ + Cl-
-
?
2,3,5-trichloromaleylacetate + NADH
-
Cupriavidus necator 2,4-dichloro-3-oxohexanedioic acid + NAD+ + Cl-
-
?
2,3-dibromomaleylacetate + NADH
-
Pseudomonas aeruginosa 3-bromo-4-oxohexanedioic acid + NAD+ + Br-
-
?
2,3-dibromomaleylacetate + NADH
-
Pseudomonas sp. 3-bromo-4-oxohexanedioic acid + NAD+ + Br-
-
?
2,3-dibromomaleylacetate + NADH
-
Cupriavidus necator 3-bromo-4-oxohexanedioic acid + NAD+ + Br-
-
?
2,3-dichloromaleylacetate + NADH
-
Pseudomonas aeruginosa 3-chloro-4-oxohexanedioic acid + NAD+ + Cl-
-
?
2,3-dichloromaleylacetate + NADH
-
Pseudomonas sp. 3-chloro-4-oxohexanedioic acid + NAD+ + Cl-
-
?
2,3-dichloromaleylacetate + NADH
-
Cupriavidus necator 3-chloro-4-oxohexanedioic acid + NAD+ + Cl-
-
?
2,5-dichloromaleylacetate + NADH
-
Pseudomonas aeruginosa 2-chloro-3-oxohexanedioic acid + NAD+ + Cl-
-
?
2,5-dichloromaleylacetate + NADH
-
Pseudomonas sp. 2-chloro-3-oxohexanedioic acid + NAD+ + Cl-
-
?
2,5-dichloromaleylacetate + NADH
-
Cupriavidus necator 2-chloro-3-oxohexanedioic acid + NAD+ + Cl-
-
?
2-bromomaleylacetate + NADH
-
Pseudomonas aeruginosa 2-maleylacetate + NAD+ + Br-
-
?
2-bromomaleylacetate + NADH
-
Cupriavidus necator 2-maleylacetate + NAD+ + Br-
-
?
2-bromomaleylacetate + NADH
-
Pseudomonas sp. 2-maleylacetate + NAD+ + Br- the product is further reduced to 2-bromo-3-oxohexanedioic acid under consumption of NADH ?
2-chloromaleylacetate + NADH
-
Pseudomonas aeruginosa 2-maleylacetate + Cl- + NAD+
-
?
2-chloromaleylacetate + NADH
-
Pseudomonas sp. 2-maleylacetate + Cl- + NAD+
-
?
2-chloromaleylacetate + NADH
-
Cupriavidus necator 2-maleylacetate + Cl- + NAD+ the product is further reduced to 3-oxoadipate under consumption of NADH ?
2-chloromaleylacetate + NADH
-
Pseudomonas aeruginosa RHO1 2-maleylacetate + Cl- + NAD+
-
?
2-chloromaleylacetate + NADH
-
Cupriavidus necator JMP 134-1 2-maleylacetate + Cl- + NAD+ the product is further reduced to 3-oxoadipate under consumption of NADH ?
2-maleylacetate + NAD(P)H
-
Pseudomonas aeruginosa 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Pseudomonas sp. 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Cupriavidus necator 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Pseudomonas aeruginosa RHO1 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NAD(P)H
-
Cupriavidus necator JMP 134-1 3-oxoadipate + NAD(P)+
-
ir
2-maleylacetate + NADPH
-
Pseudomonas aeruginosa 3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH
-
Pseudomonas sp. 3-oxoadipate + NADP+
-
ir
2-maleylacetate + NADPH
-
Cupriavidus necator 3-oxoadipate + NADP+
-
ir
2-methylmaleylacetate + NAD(P)H
-
Pseudomonas aeruginosa 5-methyl-3-oxoadipate + NAD(P)+
-
?
2-methylmaleylacetate + NAD(P)H
-
Pseudomonas sp. 5-methyl-3-oxoadipate + NAD(P)+
-
?
2-methylmaleylacetate + NAD(P)H
-
Cupriavidus necator 5-methyl-3-oxoadipate + NAD(P)+
-
?
2-methylmaleylacetate + NAD(P)H
-
Pseudomonas aeruginosa RHO1 5-methyl-3-oxoadipate + NAD(P)+
-
?
2-methylmaleylacetate + NAD(P)H
-
Cupriavidus necator JMP 134-1 5-methyl-3-oxoadipate + NAD(P)+
-
?
3-bromomaleylacetate + NADH
-
Pseudomonas aeruginosa 3-bromo-4-oxohexanedioic acid + NAD+
-
?
3-bromomaleylacetate + NADH
-
Pseudomonas sp. 3-bromo-4-oxohexanedioic acid + NAD+
-
?
3-bromomaleylacetate + NADH
-
Cupriavidus necator 3-bromo-4-oxohexanedioic acid + NAD+
-
?
3-chloromaleylacetate + NADH
-
Pseudomonas aeruginosa 3-chloro-4-oxohexanedioic acid + NAD+
-
?
3-chloromaleylacetate + NADH
-
Pseudomonas sp. 3-chloro-4-oxohexanedioic acid + NAD+
-
?
3-chloromaleylacetate + NADH
-
Cupriavidus necator 3-chloro-4-oxohexanedioic acid + NAD+
-
?
3-methylmaleylacetate + NAD(P)H
-
Pseudomonas aeruginosa 4-methyl-3-oxoadipate + NAD(P)+
-
?
3-methylmaleylacetate + NAD(P)H
-
Pseudomonas sp. 4-methyl-3-oxoadipate + NAD(P)+
-
?
3-methylmaleylacetate + NAD(P)H
-
Cupriavidus necator 4-methyl-3-oxoadipate + NAD(P)+
-
?
3-methylmaleylacetate + NAD(P)H
-
Pseudomonas aeruginosa RHO1 4-methyl-3-oxoadipate + NAD(P)+
-
?
3-methylmaleylacetate + NAD(P)H
-
Cupriavidus necator JMP 134-1 4-methyl-3-oxoadipate + NAD(P)+
-
?
5-bromomaleylacetate + NADH
-
Pseudomonas aeruginosa 2-bromo-3-oxohexanedioic acid + NAD+
-
?
5-bromomaleylacetate + NADH
-
Pseudomonas sp. 2-bromo-3-oxohexanedioic acid + NAD+
-
?
5-bromomaleylacetate + NADH
-
Cupriavidus necator 2-bromo-3-oxohexanedioic acid + NAD+
-
?
5-chloromaleylacetate + NADH
-
Pseudomonas aeruginosa 2-chloro-3-oxohexanedioic acid + NAD+
-
?
5-chloromaleylacetate + NADH
-
Pseudomonas sp. 2-chloro-3-oxohexanedioic acid + NAD+
-
?
5-chloromaleylacetate + NADH
-
Cupriavidus necator 2-chloro-3-oxohexanedioic acid + NAD+
-
?
5-fluoromaleylacetate + NADH
-
Pseudomonas aeruginosa 2-fluoro-3-oxohexanedioic acid + NAD+
-
?
5-fluoromaleylacetate + NADH
-
Cupriavidus necator 2-fluoro-3-oxohexanedioic acid + NAD+
-
?
5-methylmaleylacetate + NAD(P)H
-
Pseudomonas aeruginosa 2-methyl-3-oxoadipate + NADP+
-
?
5-methylmaleylacetate + NAD(P)H
-
Pseudomonas sp. 2-methyl-3-oxoadipate + NADP+
-
?
5-methylmaleylacetate + NAD(P)H
-
Cupriavidus necator 2-methyl-3-oxoadipate + NADP+
-
?
5-methylmaleylacetate + NAD(P)H
-
Pseudomonas aeruginosa RHO1 2-methyl-3-oxoadipate + NADP+
-
?
5-methylmaleylacetate + NAD(P)H
-
Cupriavidus necator JMP 134-1 2-methyl-3-oxoadipate + NADP+
-
?

Subunits

Subunits Comment Organism
dimer
-
Pseudomonas sp.
dimer
-
Cupriavidus necator
dimer 2 * 37000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
MAR
-
Pseudomonas aeruginosa
MAR
-
Cupriavidus necator

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
Pseudomonas aeruginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.8
-
activity drastically reduced at pH values below Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NADH
-
Pseudomonas sp.
NADH
-
Cupriavidus necator