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Literature summary for 1.3.1.32 extracted from

  • Seibert, V.; Thiel, M.; Hinner, I.S.; Schlomann, M.
    Characterization of a gene cluster encoding the maleylacetate reductase from Ralstonia eutropha 335T, an enzyme recruited for growth with 4-fluorobenzoate (2004), Microbiology, 150, 463-472.
    View publication on PubMed
Search for more literature for 1.3.1.32

Cloned(Commentary)

Cloned (Comment) Organism
gene macA, DNA sequence determination and analysis, gene cluster analysis, the cluster also comprises genes macB and macR, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, overexpression in Escherichia coli strain BL21(DE3) Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-maleylacetate + NAD(P)H Cupriavidus necator enzyme plays a crucial role in the degradation of aromatic compounds 3-oxoadipate + NAD(P)+
-
 ?
2-maleylacetate + NAD(P)H Cupriavidus necator DSM 531 enzyme plays a crucial role in the degradation of aromatic compounds 3-oxoadipate + NAD(P)+
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator Q9RBG1 strain 335, gene macA
-
Cupriavidus necator DSM 531 Q9RBG1 strain 335, gene macA
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme, over 246fold by several chromatographic steps Cupriavidus necator

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme is required for growth on 4-fluorobenzoate as sole carbon source Cupriavidus necator
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27
-
 above, purified enzyme Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloromaleylacetate + NAD(P)H
-
 Cupriavidus necator ? + NAD(P)+
-
 ?
2-chloromaleylacetate + NAD(P)H
-
 Cupriavidus necator DSM 531 ? + NAD(P)+
-
 ?
2-maleylacetate + NAD(P)H
-
 Cupriavidus necator 3-oxoadipate + NAD(P)+
-
 ?
2-maleylacetate + NAD(P)H enzyme plays a crucial role in the degradation of aromatic compounds Cupriavidus necator 3-oxoadipate + NAD(P)+
-
 ?
2-maleylacetate + NAD(P)H
-
 Cupriavidus necator DSM 531 3-oxoadipate + NAD(P)+
-
 ?
2-maleylacetate + NAD(P)H enzyme plays a crucial role in the degradation of aromatic compounds Cupriavidus necator DSM 531 3-oxoadipate + NAD(P)+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
 Cupriavidus necator