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Literature summary for 1.3.1.33 extracted from

  • Beer, N.S.; Griffiths, W.T.
    Purification of the enzyme NADPH: protochlorophyllide oxidoreductase (1981), Biochem. J., 195, 83-92.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
etioplast
-
Phaseolus vulgaris 9513
-
etioplast
-
Hordeum vulgare 9513
-
etioplast
-
Avena sativa 9513
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
-
Phaseolus vulgaris
35000
-
gel filtration Hordeum vulgare
35000
-
gel filtration Avena sativa
35000
-
SDS-PAGE shows 2 closely related peptides of 35000 and 37000 Avena sativa
37000
-
-
Hordeum vulgare
37000
-
gel filtration, doublet of 2 peptides of 35000 and 37000 co-purified Avena sativa
38000
-
gel filtration Hordeum vulgare
45000
-
2 * 45000, SDS-PAGE Phaseolus vulgaris
63000
-
-
Hordeum vulgare
100000
-
-
Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protochlorophyllide + NADPH Phaseolus vulgaris
-
chlorophyllide + NADP+
-
?
protochlorophyllide + NADPH Hordeum vulgare
-
chlorophyllide + NADP+
-
?
protochlorophyllide + NADPH Avena sativa
-
chlorophyllide + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Avena sativa
-
oat
-
Avena sativa
-
L., var. Pennal
-
Hordeum vulgare
-
barley
-
Hordeum vulgare
-
L., cultivar Proctor
-
Phaseolus vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hordeum vulgare
-
Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Phaseolus vulgaris
-
leaf
-
Hordeum vulgare
-
leaf
-
Avena sativa
-
shoot
-
Hordeum vulgare
-
shoot
-
Avena sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.5
-
etioplast membranes Hordeum vulgare
13.9
-
-
Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protochlorophyllide + NADPH
-
Phaseolus vulgaris chlorophyllide + NADP+
-
?
protochlorophyllide + NADPH
-
Hordeum vulgare chlorophyllide + NADP+
-
?
protochlorophyllide + NADPH
-
Avena sativa chlorophyllide + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 45000, SDS-PAGE Phaseolus vulgaris