Literature summary for 1.3.1.33 extracted from

Buhr, F.; El Bakkouri, M.; Valdez, O.; Pollmann, S.; Lebedev, N.; Reinbothe, S.; Reinbothe, C.
Photoprotective role of NADPH:protochlorophyllide oxidoreductase A (2008), Proc. Natl. Acad. Sci. USA, 105, 12629-12634.

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Cloned(Commentary)

Cloned (Comment)	Organism
into the vector pQE30	Hordeum vulgare

Protein Variants

Protein Variants	Comment	Organism
C195A	mutant, constructed for the identification of the protochlorophyllide binding site	Hordeum vulgare
C222A	mutant, constructed for the identification of the protochlorophyllide binding site	Hordeum vulgare
C33A	mutant, constructed for the identification of the protochlorophyllide binding site	Hordeum vulgare
C85A	mutant, constructed for the identification of the protochlorophyllide binding site	Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	determined by SDS-PAGE	Hordeum vulgare
42000	-	determined by SDS-PAGE	Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
protochlorophyllide + NADPH + H+	Hordeum vulgare	-	chlorophyllide + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Hordeum vulgare	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by Ni-NTA agarose chromatography	Hordeum vulgare

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
protochlorophyllide + NADPH + H+	-	Hordeum vulgare	chlorophyllide + NADP+	-	?

Subunits

Subunits	Comment	Organism
oligomer	oligomeric PORA-PORB complex	Hordeum vulgare

Synonyms

Synonyms	Comment	Organism
NADPH:protochlorophyllide oxidoreductase A	-	Hordeum vulgare
NADPH:protochlorophyllide oxidoreductase B	-	Hordeum vulgare
PORA	-	Hordeum vulgare
PORB	-	Hordeum vulgare

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Hordeum vulgare

Expression

Organism	Comment	Expression
Hordeum vulgare	PORA represents the negatively light-regulated POR enzyme	down

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Release 2023.1 (January 2023)