Cloned (Comment) | Organism |
---|---|
expression in strain BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
15 mg/ml purified recombinant enzyme, hanging drop vapour diffusion method, reservoir solution: 30% PEG 5000 monomethyl ether, 0.2 M sodium acetate, 0.1 M ammonium sulfate, 0.1 M 3-(N-morpholino)ethane sulfonic acid, pH 6.5, precipitant solution: 18% PEG 5000 monomethyl ether, 180 mM sodium acetate, 90 mM ammonium sulfate, 90 mM 3-(N-morpholino)ethane sulfonic acid, mixture in a ratio 1:1.5, 1 week, no cryoprotection, X-ray diffraction structure determination and analysis at 2.2 A resolution, heavy atom derivatives | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | enzyme is a iron-sulfur flavoenzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from strain BL21(DE3) via DEAE ion exchange and affinity chromatography | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a (2E)-2-enoyl-CoA + NADP+ = a (2E,4E)-2,4-dienoyl-CoA + NADPH + H+ | reaction mechanism, active site structure, substrate binding site, His252 is the catalytic residue | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
2,4-dienoyl-CoA reductase | - |
Escherichia coli |
DCR | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | enzyme is a iron-sulfur flavoenzyme | Escherichia coli |