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Literature summary for 1.3.1.37 extracted from

  • Mizugaki, M.; Unuma, T.; Yamanaka, H.
    Studies on the metabolism of unsaturated fatty acids. II. Separation and general properties of reduced nicotinamide adenine dinucleotide phosphate dependent cis-2-enoyl-coenzyme A reductase from Escherichia coli K-12 (1979), Chem. Pharm. Bull., 27, 2334-2337.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
iodoacetic acid 1 mM, 34% inhibition Escherichia coli
p-hydroxymercuribenzoate 1 mM, 77% inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 cis-2-octenoyl-CoA
-
 Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0005
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, ammonium sulfate precipitate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-2-decenoyl-CoA + NADPH + H+ no reduction of cis-3-enoyl substrates Escherichia coli decanoyl-CoA + NADP+
-
 ?
cis-2-dodecenoyl-CoA + NADPH + H+
-
 Escherichia coli dodecanoyl-CoA + NADP+
-
 ?
cis-2-octenoyl-CoA + NADPH + H+
-
 Escherichia coli octanoyl-CoA + NADP+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 no loss of activity after 30 min Escherichia coli
55
-
 10 min, inactivation Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Escherichia coli