Cloned (Comment) | Organism |
---|---|
construction of a cDNA library, phylogenetic analysis, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
x * 41000, recombinant His-tagged enzyme, SDS-PAGE | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(15Z)-12-oxophyto-10,15-dienoate + NADPH | Oryza sativa | leads to jasmonate biosynthesis | 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
additional information | Oryza sativa | physiological role of the enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q84QK0 | cv. BL-1, enzyme OsOPR1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(15Z)-12-oxophyto-10,15-dienoate + NADPH | leads to jasmonate biosynthesis | Oryza sativa | 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
(15Z)-12-oxophyto-10,15-dienoate + NADPH | preference of (-)-cis isomer over the (+)-cis-isomer | Oryza sativa | 8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+ | - |
? | |
additional information | physiological role of the enzyme | Oryza sativa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 41000, recombinant His-tagged enzyme, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
OPR | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Oryza sativa |