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Literature summary for 1.3.1.42 extracted from

  • Beynon, E.R.; Symons, Z.C.; Jackson, R.G.; Lorenz, A.; Rylott, E.L.; Bruce, N.C.
    The role of oxophytodienoate reductases in the detoxification of the explosive 2,4,6-trinitrotoluene by Arabidopsis (2009), Plant Physiol., 151, 253-261.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.42

Activating Compound

Activating Compound Comment Organism Structure
2,4,6-trinitrotoluene expression of oxophytodienoate reductases increases in response to the explosive 2,4,6-trinitrotoluene, 17fold up-regulation of OPR1 in the root Arabidopsis thaliana
2,4,6-trinitrotoluene expression of oxophytodienoate reductases increases in response to the explosive 2,4,6-trinitrotoluene, 6fold up-regulation of OPR2 in the root Arabidopsis thaliana

Application

Application Comment Organism
environmental protection 2,4,6-trinitrotoluene detoxofication, use of plants to remove environmental pollutants Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR1 Arabidopsis thaliana
recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR2 Arabidopsis thaliana
recombinant expression of OPR3 in Escherichia coli Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome OPR3 Arabidopsis thaliana 5777
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(9S,13S)-12-oxophytodienoic acid + NADPH + H+ Arabidopsis thaliana OPR3 is involved in jasmonic acid biosynthesis 3-oxo-2-((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8GYB8 ecotypes Col-0 and Wassilewskija
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Arabidopsis thaliana Q8LAH7 ecotypes Col-0 and Wassilewskija
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Arabidopsis thaliana Q9FUP0 ecotypes Col-0 and Wassilewskija
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Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Arabidopsis thaliana
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seedling OPR overexpressing line Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 OPR1-overexpressing lines have seedling root lengths significantly longer than those of wild-type plants when growing on solid medium containing 2 microM 2,4,6-trinitrotoluene, enhanced tolerance to 2,4,6-trinitrotoluene agrees with that observed in transgenic tobacco, OPR1-overexpressing lines 1.1 and 9.4 show enhanced 2,4,6-trinitrotoluene uptake Arabidopsis thaliana
additional information
-
 OPR2-overexpressing lines have seedling root lengths significantly longer than those of wild-type plants when growing on solid medium containing 2 microM 2,4,6-trinitrotoluene, enhanced tolerance to 2,4,6-trinitrotoluene agrees with that observed in transgenic tobacco Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9S,13S)-12-oxophytodienoic acid + NADPH + H+ OPR3 is involved in jasmonic acid biosynthesis Arabidopsis thaliana 3-oxo-2-((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+
-
 ?
additional information OPR1 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives, it additionally produces the aromatic ring-reduced products hydride and dihydride Meisenheimer complexes Arabidopsis thaliana ?
-
 ?
additional information OPR2 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives Arabidopsis thaliana ?
-
 ?
additional information OPR3 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
OPR
-
 Arabidopsis thaliana
OPR1
-
 Arabidopsis thaliana
OPR2
-
 Arabidopsis thaliana
OPR3
-
 Arabidopsis thaliana
oxophytodienoate reductase
-
 Arabidopsis thaliana
phytodienoic acid reductase
-
 Arabidopsis thaliana