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Literature summary for 1.3.1.45 extracted from

  • Lopez-Meyer, M.; Paiva, N.L.
    Immunolocalization of vestitone reductase and isoflavone reductase, two enzymes involved in the biosynthesis of the phytoalexin medicarpin (2002), Physiol. Mol. Plant Pathol., 61, 15-30.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme expression is induced upon fungal infection in leaves and elicitation in cell culture Medicago sativa

Cloned(Commentary)

Cloned (Comment) Organism
expression in transgenic Nicotiana tabacum plants under control of the CaMV35S promotor Medicago sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Medicago sativa 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2'-hydroxyformononetin + NADPH Medicago sativa enzyme is involved in the biosynthesis of the phytoalexin medicarpin, constitutively in roots and nodules and inducible in leaves, overview vestitone + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Medicago sativa
-
cv. Apollo
-

Purification (Commentary)

Purification (Comment) Organism
partially, subcellular fractions of cultured cells Medicago sativa

Source Tissue

Source Tissue Comment Organism Textmining
cell culture constitutive expression Medicago sativa
-
leaf enzyme expression is induced upon fungal infection Medicago sativa
-
additional information tissue and subcellular distribution in wild-type alfalfa plants and transgenic tobacco plants, overview Medicago sativa
-
root constitutive expression Medicago sativa
-
root nodule constitutive expression Medicago sativa
-
seedling
-
Medicago sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-hydroxyformononetin + NADPH
-
Medicago sativa vestitone + NADP+
-
?
2'-hydroxyformononetin + NADPH enzyme is involved in the biosynthesis of the phytoalexin medicarpin, constitutively in roots and nodules and inducible in leaves, overview Medicago sativa vestitone + NADP+
-
?

Synonyms

Synonyms Comment Organism
IFR
-
Medicago sativa
isoflavone reductase
-
Medicago sativa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Medicago sativa