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Literature summary for 1.3.1.48 extracted from

  • Hansen, H.S.
    Purification and assay of 15-ketoprostaglandin delta 13-reductase from bovine lung (1982), Methods Enzymol., 86, 156-167.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
NADP+
-
Bos taurus
p-chloromercuribenzoate
-
Bos taurus
p-chloromercuribenzoate
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
NADPH
-
Bos taurus
0.01
-
15-ketoprostaglandin E1 human placenta Homo sapiens
0.03
-
15-ketoprostaglandin E1 bovine lung Bos taurus
0.062
-
15-ketoprostaglandin E2
-
Bos taurus
0.091
-
NADH
-
Bos taurus
0.184
-
15-ketoprostaglandin F1alpha
-
Bos taurus
0.258
-
6,15-diketo-prostaglandin F1alpha
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
gel filtration Bos taurus
68500
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
15-ketoprostaglandin E + NAD(P)H Gallus gallus second step in metabolism of E- and Falpha-type prostaglandins ?
-
?
15-ketoprostaglandin E + NAD(P)H Homo sapiens second step in metabolism of E- and Falpha-type prostaglandins ?
-
?
15-ketoprostaglandin E + NAD(P)H Bos taurus second step in metabolism of E- and Falpha-type prostaglandins ?
-
?
15-oxoprostaglandin F1alpha + NAD(P)H Gallus gallus second step in metabolism of E- and Falpha-type prostaglandins ?
-
?
15-oxoprostaglandin F1alpha + NAD(P)H Homo sapiens second step in metabolism of E- and Falpha-type prostaglandins ?
-
?
15-oxoprostaglandin F1alpha + NAD(P)H Bos taurus second step in metabolism of E- and Falpha-type prostaglandins ?
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Gallus gallus
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Gallus gallus
-
lung
-
Bos taurus
-
placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.067
-
-
Bos taurus

Storage Stability

Storage Stability Organism
-4°C, 0.1 M sodium phosphate, pH 7.4, 1 mM EDTA, 1 mM mercaptoethanol, stable for more than 1 month Bos taurus
-80°C, stable for more than 1 year Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H
-
Gallus gallus (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
ir
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H
-
Homo sapiens (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
ir
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H
-
Bos taurus (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+
-
ir
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H i.e. 15-ketoprostaglandin E2 Homo sapiens 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
?
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H i.e. 15-ketoprostaglandin E2 Bos taurus 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+
-
?
15-ketoprostaglandin E + NAD(P)H second step in metabolism of E- and Falpha-type prostaglandins Gallus gallus ?
-
?
15-ketoprostaglandin E + NAD(P)H second step in metabolism of E- and Falpha-type prostaglandins Homo sapiens ?
-
?
15-ketoprostaglandin E + NAD(P)H second step in metabolism of E- and Falpha-type prostaglandins Bos taurus ?
-
?
15-ketoprostaglandin E1 + NADH + H+
-
Bos taurus 13,14-dihydro-15-keto-prostaglandin E1 + NAD+
-
r
15-ketoprostaglandin E1 + NADPH
-
Homo sapiens ?
-
?
15-ketoprostaglandin E1 + NADPH
-
Bos taurus ?
-
?
15-ketoprostaglandin F1alpha + NAD(P)H
-
Bos taurus ?
-
?
15-oxoprostaglandin F1alpha + NAD(P)H second step in metabolism of E- and Falpha-type prostaglandins Gallus gallus ?
-
?
15-oxoprostaglandin F1alpha + NAD(P)H second step in metabolism of E- and Falpha-type prostaglandins Homo sapiens ?
-
?
15-oxoprostaglandin F1alpha + NAD(P)H second step in metabolism of E- and Falpha-type prostaglandins Bos taurus ?
-
?
6,15-diketo-prostaglandin F1alpha + NAD(P)H
-
Bos taurus ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
Homo sapiens
NAD(P)H bovine lung: enzyme can use both NADPH and NADH Bos taurus
NADH human placenta: enzyme can use only NADH Homo sapiens