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Literature summary for 1.3.1.48 extracted from

  • Chou, W.L.; Chuang, L.M.; Chou, C.C.; Wang, A.H.; Lawson, J.A.; FitzGerald, G.A.; Chang, Z.F.
    Identification of a novel prostaglandin reductase reveals the involvement of prostaglandin E2 catabolism in regulation of peroxisome proliferator-activated receptor gamma activation (2007), J. Biol. Chem., 282, 18162-18172.
    View publication on PubMed
Search for more literature for 1.3.1.48

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain XL1-Blue Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0344
-
 15-ketoprostaglandin E1 in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
0.0496
-
 15-ketoprostaglandin E2 in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
0.0592
-
 15-ketoprostaglandin F1alpha in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
0.0946
-
 NADPH in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
0.1088
-
 15-ketoprostaglandin F2alpha in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus Q8VDQ1
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
3T3-L1 cell
-
 Mus musculus
-
adipocyte
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.115
-
 using 15-ketoprostaglandin E1 as a substrate, at 37°C Mus musculus
0.1784
-
 using 15-ketoprostaglandin E2 as a substrate, at 37°C Mus musculus
0.2064
-
 using 15-ketoprostaglandin F1alpha as a substrate, at 37°C Mus musculus
0.2309
-
 using 15-ketoprostaglandin F2alpha as a substrate, at 37°C Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
15-ketoprostaglandin E1 + NADPH
-
 Mus musculus ?
-
 ?
15-ketoprostaglandin E2 + NADPH + H+
-
 Mus musculus 13,14-dihydro-15-keto-prostaglandin E2 + NADP+
-
 ?
15-ketoprostaglandin F1alpha + NADPH
-
 Mus musculus ?
-
 ?
15-ketoprostaglandin F2alpha + NADPH
-
 Mus musculus ?
-
 ?
additional information not active towards 6-keto-prostaglandin F1alpha, prostaglandin F2beta, 11beta-prostaglandin F2alpha, 13,14-dihydro-15-keto-prostaglandin D2, 13,14-dihydro-15-keto-prostaglandin E2, 13,14-dihydro-15-keto-PGF2alpha, and leukotriene B4 Mus musculus ?
-
 ?

Synonyms

Synonyms Comment Organism
15-oxoprostaglandin-DELTA13-reductase
-
 Mus musculus
PGR-2
-
 Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 15-ketoprostaglandin E1 in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
0.16
-
 NADPH in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
0.19
-
 15-ketoprostaglandin E2 in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
0.22
-
 15-ketoprostaglandin F1alpha in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus
0.25
-
 15-ketoprostaglandin F2alpha in 0.1 M Tris-HCl (pH 7.4), at 37°C Mus musculus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Mus musculus