Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4,6-Trihydroxybenzoate | tested at 1.0 mM and under N2, 49% inhibition | Eubacterium oxidoreducens | |
6-Hydroxynicotinate | tested at 1.0 mM, 12% inhibition | Eubacterium oxidoreducens | |
catechol | tested at 1.0 mM, 14% inhibition | Eubacterium oxidoreducens | |
CuCl2 | tested at 0.1 mM, 100% inhibition | Eubacterium oxidoreducens | |
diethyldicarbonate | tested at 1.0 mM, 54% inhibition | Eubacterium oxidoreducens | |
gallate | tested at 1.0 mM and under N2, 24% inhibition | Eubacterium oxidoreducens | |
hydroquinone | tested at 1.0 mM and under N2, 31% inhibition | Eubacterium oxidoreducens | |
menadione | tested at 1.0 mM, 33% inhibition | Eubacterium oxidoreducens | |
N-ethylmaleimide | tested at 1.0 mM, 94% inhibition | Eubacterium oxidoreducens | |
p-chloromercuribenzoate | tested at 0.1 mM, 100% inhibition | Eubacterium oxidoreducens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
NADPH | at pH 6.8 | Eubacterium oxidoreducens | |
0.027 | - |
NADP+ | reverse direction at pH 7.2 | Eubacterium oxidoreducens | |
0.29 | - |
dihydrophloroglucinol | reverse direction at pH 7.2 | Eubacterium oxidoreducens | |
0.56 | - |
phloroglucinol | at pH 7.6 | Eubacterium oxidoreducens | |
0.6 | - |
phloroglucinol | at pH 7.2 | Eubacterium oxidoreducens | |
0.8 | - |
phloroglucinol | at pH 6.8 | Eubacterium oxidoreducens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * alpha, 2 * 33000, gel electrophoresis | Eubacterium oxidoreducens |
78000 | - |
gel filtration chromatography with native enzyme | Eubacterium oxidoreducens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phloroglucinol + NADPH | Eubacterium oxidoreducens | pathway of anaerobic degradation of trihydroxybenzenes and metabolism of gallic acid | dihydrophloroglucinol + NADP | - |
r | |
phloroglucinol + NADPH | Eubacterium oxidoreducens G-41 | pathway of anaerobic degradation of trihydroxybenzenes and metabolism of gallic acid | dihydrophloroglucinol + NADP | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eubacterium oxidoreducens | - |
- |
- |
Eubacterium oxidoreducens G-41 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
prepared of a cell extract by French press, DEAE-cellulose column, phenyl-Sepharose column, ultrafiltration, red agarose column, anion exchange column, purified 90.4fold with a yield of 29% | Eubacterium oxidoreducens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
rumen | organism is a strict anaerobe | Eubacterium oxidoreducens | - |
Storage Stability | Organism |
---|---|
stable for several hours when kept on ice and retained full activity being frozen with 15% glycerol in liquid N2. Incubation at 30°C and pH 7.0, 82% of initial activity retained after 3.3 h | Eubacterium oxidoreducens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phloroglucinol + NADPH | - |
Eubacterium oxidoreducens | dihydrophloroglucinol + NADP+ | - |
r | |
phloroglucinol + NADPH | - |
Eubacterium oxidoreducens G-41 | dihydrophloroglucinol + NADP+ | - |
r | |
phloroglucinol + NADPH | pathway of anaerobic degradation of trihydroxybenzenes and metabolism of gallic acid | Eubacterium oxidoreducens | dihydrophloroglucinol + NADP | - |
r | |
phloroglucinol + NADPH | pathway of anaerobic degradation of trihydroxybenzenes and metabolism of gallic acid | Eubacterium oxidoreducens G-41 | dihydrophloroglucinol + NADP | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * alpha, 2 * 33000, gel electrophoresis | Eubacterium oxidoreducens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Eubacterium oxidoreducens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Eubacterium oxidoreducens |