Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | up to 17fold increase in methyl viologen dependent activity, loss of NADH dependent activity | Ruminobacter amylophilus |
General Stability | Organism |
---|---|
inactivation by irradiation at 254 nm | Ruminobacter amylophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-n-heptyl-4-hydroxyquinoline-N-oxide | strong inhibitor | Ruminobacter amylophilus | |
Acriflavin | specific inhibition of NADH dependent activity | Ruminobacter amylophilus | |
ClHgSO3H | inhibition with all cofactors except the methyl viologen dependent activity | Ruminobacter amylophilus | |
KCN | inhibition with all cofactors except the methyl viologen dependent activity | Ruminobacter amylophilus | |
additional information | inactivation by irradiation at 254 nm, can be partially restored by addition of 2,3-dimethyl-1,4-naphthoquinone | Ruminobacter amylophilus | |
Triton X-100 | complete loss of NADH dependent activity at concentration of 0.05%, up to 17fold increase in methyl viologen dependent activity | Ruminobacter amylophilus | |
Zn2+ | strong inhibitor | Ruminobacter amylophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADH | - |
Ruminobacter amylophilus | |
0.025 | - |
fumarate | - |
Ruminobacter amylophilus | |
0.086 | - |
FMNH2 | - |
Ruminobacter amylophilus | |
0.28 | - |
FADH2 | - |
Ruminobacter amylophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Ruminobacter amylophilus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Na+ | slight activation | Ruminobacter amylophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumarate + NADH + H+ | Ruminobacter amylophilus | - |
succinate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminobacter amylophilus | - |
- |
- |
Oxidation Stability | Organism |
---|---|
sensitive to air oxidation, loss of activity | Ruminobacter amylophilus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.14 | 0.25 | with NADH, FMNH2 and FADH2 | Ruminobacter amylophilus |
0.84 | - |
with benzyl- or mehtyl viologen | Ruminobacter amylophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fumarate + FADH2 | - |
Ruminobacter amylophilus | succinate + FAD | - |
r | |
fumarate + FMNH2 | - |
Ruminobacter amylophilus | succinate + FMN | - |
r | |
fumarate + NADH + H+ | - |
Ruminobacter amylophilus | succinate + NAD+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | - |
Ruminobacter amylophilus | |
FMNH2 | - |
Ruminobacter amylophilus | |
NADH | - |
Ruminobacter amylophilus |