Literature summary for 1.3.1.6 extracted from

Coustou, V.; Besteiro, S.; Riviere, L.; Biran, M.; Biteau, N.; Franconi, J.M.; Boshart, M.; Baltz, T.; Bringaud, F.
A mitochondrial NADH-dependent fumarate reductase involved in the production of succinate excreted by procyclic Trypanosoma brucei (2005), J. Biol. Chem., 280, 16559-16570.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene FRDg, DNA sequence determination and analysis, gene expression inhibition wby RNAi, stem-loop sense/antisense molecules, expresssion of EGFP-tagged enzyme in trypanosomes, cell line EATRO1125.T7T	Trypanosoma brucei
gene FRDm1, DNA sequence determination and analysis, gene expression inhibition wby RNAi, stem-loop sense/antisense molecules, expresssion of EGFP-tagged enzyme in trypanosomes, cell line EATRO1125.T7T	Trypanosoma brucei
gene FRDm2, DNA sequence determination and analysis, gene expression inhibition wby RNAi, stem-loop sense/antisense molecules, expresssion of EGFP-tagged enzyme in trypanosomes, cell line EATRO1125.T7T	Trypanosoma brucei

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
glycosome	soluble enzyme FRDg	Trypanosoma brucei	20015	-
mitochondrion	isozymes FRDm1 and FRDm2	Trypanosoma brucei	5739	-

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	Q5BU12	isozyme FRDm2; soluble glycosomal and mitochondrial enzyme forms, the latter as 2 isozymes	-
Trypanosoma brucei	Q5BU14	isozyme FRDm1; soluble glycosomal and mitochondrial enzyme forms, the latter as 2 isozymes	-
Trypanosoma brucei	Q8ITL2	isozyme FRDg; soluble glycosomal and mitochondrial enzyme forms, the latter as 2 isozymes	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
trypanosomoid form	procyclic	Trypanosoma brucei	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Trypanosoma brucei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
fumarate + NADH + H+	-	Trypanosoma brucei	succinate + NAD+	-	?

Synonyms

Synonyms	Comment	Organism
FRDg	-	Trypanosoma brucei
FRDm1	-	Trypanosoma brucei
FRDm2	-	Trypanosoma brucei
NADH-dependent fumarate reductase	-	Trypanosoma brucei

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Trypanosoma brucei

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Trypanosoma brucei

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Release 2023.1 (January 2023)