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Literature summary for 1.3.1.6 extracted from
- The essential role of fumarate reductase in haem-dependent growth stimulation of Bacteroides fragilis (2003), Microbiology, 149, 1551-1558.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | heme induces enzyme expression and is required for optimal anaerobic growth | Bacteroides fragilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes frdA, frdB, and frdC, organized in an operon, DNA sequence determination and analysis, subcloning in Escherichia coli, expression of mutant enzymes | Bacteroides fragilis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of in frame-deletions mutants of frdC and frdB, mutation results in mutants with no catalytic activity and threefold increased generation time compared to the wild-type strain, growth can partially be restored by addition of succinate to the medium | Bacteroides fragilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | heme cofactor | Bacteroides fragilis |  |
| Mg2+ | - |
Bacteroides fragilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fumarate + NADH + H+ | Bacteroides fragilis | enzyme is required for optimal growth in presence of heme, enzyme plays a role in the generation of metabolic energy | succinate + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides fragilis | Q7X478 | iron-sulfur cluster protein subunit | - |
| Bacteroides fragilis | Q7X479 | flavoprotein subunit | - |
| Bacteroides fragilis | Q7X480 | cytochrome b subunit | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26 | - |
wild-type strain, assay under anaerobic conditions | Bacteroides fragilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fumarate + NADH + H+ | - |
Bacteroides fragilis | succinate + NAD+ | - |
? | |
| fumarate + NADH + H+ | enzyme is required for optimal growth in presence of heme, enzyme plays a role in the generation of metabolic energy | Bacteroides fragilis | succinate + NAD+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FRD | - |
Bacteroides fragilis |
| fumarate reductase | - |
Bacteroides fragilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Bacteroides fragilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | induces enzyme expression and is required for optimal anaerobic growth | Bacteroides fragilis | |
| NADH | - |
Bacteroides fragilis | |
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