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Literature summary for 1.3.1.7 extracted from

  • Kohn, L.D.; Jakoby, W.B.
    L- and mesotartaric acid dehydrogenase (crystalline) (1966), Methods Enzymol., 9, 236-240.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
mercaptan
-
Pseudomonas putida

Crystallization (Commentary)

Crystallization (Comment) Organism
rectangular prisms Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
NAD+
-
Pseudomonas putida
0.15
-
NAD+
-
Pseudomonas putida
0.45
-
meso-tartrate
-
Pseudomonas putida
7.2
-
L(+)-Tartrate
-
Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus norvegicus 5739
-
mitochondrion
-
Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
meso-tartrate + NAD+ Pseudomonas sp.
-
dihydroxyfumaric acid + NADH + H+
-
r
meso-tartrate + NAD+ Pseudomonas putida
-
dihydroxyfumaric acid + NADH + H+
-
r
meso-tartrate + NAD+ Pseudomonas sp. A
-
dihydroxyfumaric acid + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Bos taurus
-
cow
-
Pseudomonas putida
-
-
-
Pseudomonas sp.
-
A
-
Pseudomonas sp. A
-
A
-
Rattus norvegicus
-
rat
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.354
-
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyfumaric acid + NADH
-
Pseudomonas putida meso-tartrate + NAD+
-
r
L-(+)-tartrate + NAD+
-
Pseudomonas putida (3R)-oxaloglycolate + NADH
-
?
meso-tartrate + NAD+
-
Pseudomonas sp. dihydroxyfumaric acid + NADH + H+
-
r
meso-tartrate + NAD+
-
Pseudomonas putida dihydroxyfumaric acid + NADH + H+
-
r
meso-tartrate + NAD+
-
Pseudomonas sp. A dihydroxyfumaric acid + NADH + H+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
dihydroxyfumarate and NADH as substrate Pseudomonas putida
8.5
-
50% of optimal activity at pH 7 and 9 Pseudomonas putida