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Literature summary for 1.3.1.7 extracted from
- Substrate determinants of the course of tartrate dehydrogenase-catalyzed reactions (1995), Biochemistry, 34, 7517-7524.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene encoding TDH cloned into the pET3a expression vector | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
(2R,3S)-3-bromomalate | - |
Escherichia coli |  |
| 0.012 | - |
(2R,3S)-3-chloromalate | - |
Escherichia coli | |
| 0.019 | - |
(2R,3S)-3-fluoromalate | - |
Escherichia coli | |
| 0.024 | - |
(2R,3R)-3-iodomalate | - |
Escherichia coli | |
| 0.027 | - |
(2R,3S)-3-iodomalate | - |
Escherichia coli | |
| 0.05 | - |
D-malate | - |
Escherichia coli | |
| 0.06 | - |
meso-tartrate | - |
Escherichia coli | |
| 0.06 | - |
(2R,3R)-3-methyltartrate | - |
Escherichia coli | |
| 0.07 | - |
(2R,3S)-3-methyltartrate | - |
Escherichia coli | |
| 0.1 | - |
(+)-tartrate | - |
Escherichia coli | |
| 0.22 | - |
(2R,3R)-3-bromomalate | - |
Escherichia coli | |
| 0.27 | - |
(2R,3R)-3-chloromalate | - |
Escherichia coli | |
| 0.67 | - |
(2R,3R)-3-fluoromalate | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Pseudomonas putida | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2R,3R)-3-bromomalate + NAD+ | oxidative decarboxylation | Escherichia coli | ? + CO2 + NADH | - |
? | |
| (2R,3R)-3-chloromalate + NAD+ | oxidative decarboxylation | Escherichia coli | ? + CO2 + NADH | - |
? | |
| (2R,3R)-3-fluoromalate + NAD+ | oxidative decarboxylation | Escherichia coli | ? + CO2 + NADH | - |
? | |
| (2R,3R)-3-iodomalate + NAD+ | oxidative decarboxylation | Escherichia coli | ? + CO2 + NADH | - |
? | |
| (2R,3R)-3-methyltartrate + NAD+ | oxidative decarboxylation | Escherichia coli | ? + CO2 + NADH | - |
? | |
| (2R,3R)-3-thiomalate + NAD+ | - |
Escherichia coli | ? | - |
? | |
| (2R,3S)-3-bromomalate + NAD+ | oxidative decarboxylation | Escherichia coli | ? + CO2 + NADH | - |
? | |
| (2R,3S)-3-chloromalate + NAD+ | oxidative decarboxylation | Escherichia coli | ? + CO2 + NADH | - |
? | |
| (2R,3S)-3-fluoromalate + NAD+ | oxidative decarboxylation | Escherichia coli | ? + CO2 + NADH | - |
? | |
| (2R,3S)-3-iodomalate + NAD+ | oxidative decarboxylation | Escherichia coli | ? + CO2 + NADH | - |
? | |
| (2R,3S)-3-methyltartrate + NAD+ | simple oxidation | Escherichia coli | ? + NADH | - |
? | |
| (2R,3S)-3-thiomalate + NAD+ | net non-oxidative decarboxylation | Escherichia coli | ? | - |
? | |
| D-malate + NAD+ | - |
Escherichia coli | pyruvate + CO2 + NADH | - |
r | |
| L-(+)-tartrate + NAD+ | - |
Pseudomonas putida | (3R)-oxaloglycolate + NADH | - |
? | |
| L-(+)-tartrate + NAD+ | in presence of Mn2+ and K+ (+)-tartrate is subject to NAD+-dependent oxidation to form oxaloglycolate | Escherichia coli | (3R)-oxaloglycolate + NADH | - |
? | |
| malate + NAD+ | - |
Escherichia coli | pyruvate + CO2 + NADH + H+ | - |
? | |
| meso-tartrate + NAD+ | - |
Escherichia coli | D-glycerate + CO2 + NADH + H+ | - |
? | |
| additional information | 3R-oxaloglycolate, (2R,3S)-3-aminomalate and (2R,3R)-3-aminomalate are no substrates | Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tartrate dehydrogenase | - |
Escherichia coli |
| TDH | - |
Escherichia coli |
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