Application | Comment | Organism |
---|---|---|
agriculture | target for important antifungal agents for use in the control of plant diseases | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
15-aza-24-methylene-D-homocholestadiene-3beta-ol | A25822B, extremely potent inhibitor | Embryophyta | |
15-aza-24-methylene-D-homocholestadiene-3beta-ol | A25822B, extremely potent inhibitor | Zea mays | |
15-azasterol | antimycotic agent, IC50: 0.00003 mM | Embryophyta | |
15-azasterol | antimycotic agent, IC50: 0.00003 mM | Zea mays | |
8-aza-4alpha,10-dimethyl-trans-decal-3beta-ol | extremely potent inhibitor | Embryophyta | |
8-aza-4alpha,10-dimethyl-trans-decal-3beta-ol | extremely potent inhibitor | Zea mays | |
cyanide | no inhibition | Embryophyta | |
cyanide | no inhibition | Zea mays | |
fenpropidin | good inhibitor, IC50: 0.003 mM | Zea mays | |
fenpropimorph | very good inhibitor, IC50: 0.0008 mM | Zea mays | |
additional information | inhibition by a series of ammonium-ion-containing fungicides | Embryophyta | |
additional information | inhibition by a series of ammonium-ion-containing fungicides; no inhibition by 4alpha,10-dimethyl-trans-decal-3beta-ol; no inhibition by menadione or 10 mM NAD+ | Zea mays | |
N(1,5,9-trimethyldecyl)-4alpha,10-dimethyl-8-aza-trans-decal-3beta-ol | extremely potent inhibitor | Embryophyta | |
N(1,5,9-trimethyldecyl)-4alpha,10-dimethyl-8-aza-trans-decal-3beta-ol | extremely potent inhibitor | Zea mays | |
N-alkyl morpholine derivatives | fungicides | Zea mays | |
N-alkyl-8-aza-4alpha,10-dimethyl-trans-decal-3beta-ol | strong inhibition | Embryophyta | |
N-alkyl-8-aza-4alpha,10-dimethyl-trans-decal-3beta-ol | strong inhibition | Zea mays | |
N-Benzyl-8-aza-4alpha,10-dimethyl-trans-decal-3beta-ol | extremely potent inhibitor | Embryophyta | |
N-Benzyl-8-aza-4alpha,10-dimethyl-trans-decal-3beta-ol | extremely potent inhibitor | Zea mays | |
N-dodecyl-8-aza-4alpha,10-dimethyl-trans-decal-3beta-ol | extremely potent inhibitor | Embryophyta | |
N-dodecyl-8-aza-4alpha,10-dimethyl-trans-decal-3beta-ol | extremely potent inhibitor | Zea mays | |
N-substituted 8-azadecalins | potent inhibitors | Zea mays | |
N-[(3)-4-tert-butylphenyl-(2-methyl)propyl]-8-aza-4alpha,10-dimethyl-trans-decal-3beta-ol | extremely potent inhibitor | Embryophyta | |
N-[(3)-4-tert-butylphenyl-(2-methyl)propyl]-8-aza-4alpha,10-dimethyl-trans-decal-3beta-ol | extremely potent inhibitor | Zea mays | |
NADP+ | in the absence of NADPH-regenerating system, Ki: 0.45 mM; no inhibition by NAD+ | Zea mays | |
trans-1,4-bis(2-chlorobenzylaminomethyl)cyclohexane dihydrochloride | AY 9944, very slight inhibition, IC50: 0.04 mM | Zea mays | |
tridemorph | slight inhibition, IC50: 0.025 | Zea mays | |
Tween 80 | concentrations of Tween 80 higher than 1.5 g/l: inhibitory effect | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Zea mays | |
additional information | - |
additional information | kinetics | Embryophyta | |
additional information | - |
additional information | kinetic data | Zea mays | |
0.023 | - |
NADPH | substrate: 8,14-cholestadien-3beta-ol | Zea mays | |
0.1 | - |
4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol | - |
Zea mays | |
0.45 | - |
5alpha-cholesta-8,14-dien-3beta-ol | - |
Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Zea mays | 16020 | - |
membrane | membrane-bound | Embryophyta | 16020 | - |
microsome | - |
Zea mays | - |
- |
microsome | - |
Embryophyta | - |
- |
additional information | - |
Zea mays | - |
- |
additional information | - |
Embryophyta | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4alpha-methyl-8,14,24(28)-ergostatrien-3beta-ol + NADPH | Zea mays | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol | 4alpha-methyl-8,24(28)-ergostadien-3beta-ol + NADP+ | - |
? | |
4alpha-methyl-8,14,24(28)-ergostatrien-3beta-ol + NADPH | Embryophyta | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol | 4alpha-methyl-8,24(28)-ergostadien-3beta-ol + NADP+ | - |
? | |
additional information | Zea mays | DELTA8,14 derivatives are the only substrates to be transformed under normal biosynthetic conditions | ? | - |
? | |
additional information | Zea mays | enzyme involved in sterol, phytosterol, biosynthesis, DELTA8,14-sterols are intermediates in the biosynthesis of higher plant sterols, phytosterols | ? | - |
? | |
additional information | Embryophyta | enzyme involved in sterol, phytosterol, biosynthesis, DELTA8,14-sterols are intermediates in the biosynthesis of higher plant sterols, phytosterols | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Embryophyta | - |
higher plants | - |
Zea mays | - |
maize | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H+ | mechanism | Zea mays | |
4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H+ | mechanism | Embryophyta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + NADPH + H+ | - |
Zea mays | 4alpha-methylfecosterol + NADP+ | - |
? | |
4alpha-methyl-8,14,24(28)-ergostatrien-3beta-ol + NADPH | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol | Zea mays | 4alpha-methyl-8,24(28)-ergostadien-3beta-ol + NADP+ | - |
? | |
4alpha-methyl-8,14,24(28)-ergostatrien-3beta-ol + NADPH | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol | Embryophyta | 4alpha-methyl-8,24(28)-ergostadien-3beta-ol + NADP+ | - |
? | |
5alpha-cholesta-8,14-dien-3beta-ol + NADPH | DELTA8,14-cholestadien-3beta-ol | Zea mays | 5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
5alpha-cholesta-8,14-dien-3beta-ol + NADPH | DELTA8,14-cholestadien-3beta-ol | Embryophyta | 5alpha-cholesta-8-en-3beta-ol + NADP+ | - |
? | |
cholesta-8,14-dien-3beta-ol + NADPH | - |
Zea mays | cholesta-8-en-3beta-ol + NADP+ | - |
? | |
cholesta-8,14-dien-3beta-ol + NADPH | - |
Embryophyta | cholesta-8-en-3beta-ol + NADP+ | - |
? | |
additional information | substrate specificity | Zea mays | ? | - |
? | |
additional information | substrate specificity | Embryophyta | ? | - |
? | |
additional information | enzyme acts on a range of steroids with a 14(15)-double bond | Zea mays | ? | - |
? | |
additional information | enzyme acts on a range of steroids with a 14(15)-double bond | Embryophyta | ? | - |
? | |
additional information | DELTA8,14 derivatives are the only substrates to be transformed under normal biosynthetic conditions | Zea mays | ? | - |
? | |
additional information | no activity with 5alpha-cholesta-7,14-dien-3beta-ol, 7,14-cholestadien-3beta-ol or 5alpha-cholest-14-en-3beta-ol, 14-cholesten-3beta-ol | Zea mays | ? | - |
? | |
additional information | important roles of 4alpha-methyl group and side-chain substitution for recognition but not for catalysis, presence of DELTA8(9) unsaturation is compulsory for catalysis | Zea mays | ? | - |
? | |
additional information | enzyme involved in sterol, phytosterol, biosynthesis, DELTA8,14-sterols are intermediates in the biosynthesis of higher plant sterols, phytosterols | Zea mays | ? | - |
? | |
additional information | enzyme involved in sterol, phytosterol, biosynthesis, DELTA8,14-sterols are intermediates in the biosynthesis of higher plant sterols, phytosterols | Embryophyta | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at, anaerobically | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at, aerobically | Zea mays |
7.8 | - |
- |
Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
DELTA8,14-streol DELTA14-reductase has relatively broad pH profile in the basic region with optimal pH at pH 7.8, activity decreases rapidly at pH below 7 | Zea mays |
6.5 | 9 | - |
Zea mays |
7 | - |
DELTA8,14-streol DELTA14-reductase has relatively broad pH profile in the basic region with optimal pH at pH 7.8, activity decreases rapidly at pH below 7 | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | cofactor requirements | Zea mays | |
additional information | NADH does not support reduction | Zea mays | |
additional information | NADH does not support reduction | Embryophyta | |
NADPH | fully NADPH-dependent, NADH does not support reduction | Zea mays | |
NADPH | fully NADPH-dependent, NADH does not support reduction | Embryophyta |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00003 | - |
antimycotic agent, IC50: 0.00003 mM | Zea mays | 15-azasterol | |
0.00003 | - |
antimycotic agent, IC50: 0.00003 mM | Embryophyta | 15-azasterol | |
0.0008 | - |
very good inhibitor, IC50: 0.0008 mM | Zea mays | fenpropimorph | |
0.003 | - |
good inhibitor, IC50: 0.003 mM | Zea mays | fenpropidin | |
0.04 | - |
AY 9944, very slight inhibition, IC50: 0.04 mM | Zea mays | trans-1,4-bis(2-chlorobenzylaminomethyl)cyclohexane dihydrochloride |