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Literature summary for 1.3.1.70 extracted from

  • Prakash, A.; Kasbekar, D.P.
    The sterol C-14 reductase encoded by the Neurospora crassa erg-3 gene: essential charged and polar residues identified by site-specific mutagenesis (2002), Mol. Genet. Genomics, 266, 787-795.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Neurospora crassa

Protein Variants

Protein Variants Comment Organism
D207N site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
D227N site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
D276N site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
D289E site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
D289N site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
D293N site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
D394N site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
D462N site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
D463N site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
E22Q site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
E233D site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
E233Q site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
E475Q site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
H388A site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
H457A site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
K229A site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
K351A site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
K351R site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
K469A site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
R212A site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
R217A site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
R235A site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
R235K site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
R345A site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
R355A site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
R458A site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
R458K site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
R461A site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
R461K site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
T371A site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
Y124F site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
Y273F site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
Y391F site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
Y445F site-directed mutagenesis, mutant cannot complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
Y447F site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
Y470F site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
Y477F site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa
Y490F site-directed mutagenesis, mutant can complement enzyme-deficient erg-3 mutant strain mat a Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
A25822B competitive inhibitor Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Neurospora crassa enzyme plays a key role in sterol biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
gene erg-3, strain 74-OR23-1 mat, and enzyme-deficient mutant strains OR8-1 mat a and erg-3 mat a
-

Reaction

Reaction Comment Organism Reaction ID
4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP+ = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H+ determination of residues critical for activity, overview Neurospora crassa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Neurospora crassa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme plays a key role in sterol biosynthesis Neurospora crassa ?
-
?
additional information the charge and geometry given via residues E233, R235, D289, K351, R458, and R461 is important for enzyme activity Neurospora crassa ?
-
?

Synonyms

Synonyms Comment Organism
sterol C-14 reductase
-
Neurospora crassa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Neurospora crassa