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Literature summary for 1.3.1.74 extracted from
- NADPH alkenal/one oxidoreductase activity determines sensitivity of cancer cells to the chemotherapeutic alkylating agent irofulven (2004), Clin. Cancer Res., 10, 1492-1499.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in human 293 cells increases their sensitivity to irofulven by 100fold compared to control human 293 cells | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| illudin M | - |
Rattus norvegicus |  |
| illudin S | - |
Rattus norvegicus | |
| irofulven | recombinant human 293 cells show 100fold increased sensitivity compared to the wild-type human 293 cells | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.109 | - |
illudin M | recombinant enzyme, pH 7.4, 37°C | Rattus norvegicus | |
| 0.145 | - |
irofulven | recombinant enzyme, pH 7.4, 37°C | Rattus norvegicus | |
| 0.308 | - |
illudin S | recombinant enzyme, pH 7.4, 37°C | Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| illudin M + NADPH | Rattus norvegicus | extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme | ? | - |
? | |
| illudin S + NADPH | Rattus norvegicus | extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme | ? | - |
? | |
| irofulven + NADPH | Rattus norvegicus | less cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.275 | - |
purified recombinant enzyme, substrate irofulven | Rattus norvegicus |
| 44.1 | - |
purified recombinant enzyme, substrate illudin M | Rattus norvegicus |
| 115.9 | - |
purified recombinant enzyme, substrate illudin S | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| illudin M + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| illudin M + NADPH | extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme | Rattus norvegicus | ? | - |
? | |
| illudin S + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| illudin S + NADPH | extremly cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme | Rattus norvegicus | ? | - |
? | |
| irofulven + NADPH | - |
Rattus norvegicus | ? | - |
? | |
| irofulven + NADPH | less cytotoxic chemotherapeutic agent in cancer therapy, rapid metabolization by the enzyme | Rattus norvegicus | ? | - |
? | |
| additional information | reaction product identification by mass spectrometry | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AOR | - |
Rattus norvegicus |
| NADPH alkenal/one oxidoreductase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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