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Literature summary for 1.3.1.8 extracted from

  • Strom, K.A.; Kumar, S.
    Activation and inhibition of crotonyl-coenzyme A reductase activity of bovine mammary fatty acid synthetase (1979), J. Biol. Chem., 254, 8159-8162.
No PubMed abstract available
Search for more literature for 1.3.1.8

Activating Compound

Activating Compound Comment Organism Structure
coenzyme A free form, strong activator of the reduction Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
2-methylcrotonyl-CoA CoA-activated enzyme, competitive inhibition Bos taurus
3-methylcrotonyl-CoA
-
 Bos taurus
4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole
-
 Bos taurus
5,5'-dithiobis(2-nitrobenzoate)
-
 Bos taurus
acetyl-CoA competitive Bos taurus
butyryl-CoA competitive Bos taurus
coenzyme A in free form inhibits fatty acid sythesis Bos taurus
malonyl-CoA non-competitive Bos taurus
methylmalonyl-CoA i.e. tiglyl-CoA Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland
-
 Bos taurus
-

Storage Stability

Storage Stability Organism
2-3°C, absence of dithiothreitol, half life 3 h Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + NAD(P)H
-
 Bos taurus butanoyl-CoA + NADP+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 above pH 8.0, effect of CoA on pH-profile Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
6 8.3 with CoA changed from sigmoidal curve to flat one Bos taurus

Cofactor

Cofactor Comment Organism Structure
NADPH inactive with NADH Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0029
-
 2-methylcrotonyl-CoA
-
 Bos taurus
0.005
-
 butyryl-CoA
-
 Bos taurus
0.006
-
 acetyl-CoA
-
 Bos taurus
0.021
-
 malonyl-CoA
-
 Bos taurus