Literature summary for 1.3.1.82 extracted from

Ringer, K.L.; McConkey, M.E.; Davis, E.M.; Rushing, G.W.; Croteau, R.
Monoterpene double-bond reductases of the (-)-menthol biosynthetic pathway: isolation and characterization of cDNAs encoding (-)-isopiperitenone reductase and (+)-pulegone reductase of peppermint (2003), Arch. Biochem. Biophys., 418, 80-92.

Search for more literature for 1.3.1.82

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, functional expression of wild-type enzyme in Escherichia coli strain BL21(DE3), expression of a mutant cloning artifact that has a seven residue deletion at the C-terminus and bores an additional 33 vector-derived residues	Mentha x piperita

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.001	-	(-)-isopiperitenone	pH 5.5, recombinant enzyme	Mentha x piperita
0.0022	-	NADPH	pH 5.5, recombinant enzyme	Mentha x piperita

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34409	-	2 * 34409, sequence calculation, 2 * 38000, SDS-PAGE	Mentha x piperita
38000	-	2 * 34409, sequence calculation, 2 * 38000, SDS-PAGE	Mentha x piperita
60000	-	about, gel filtration	Mentha x piperita

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(-)-isopiperitenone + NADPH + H+	Mentha x piperita	pathways of monoterpene biosynthesis, overview	(+)-cis-isopulegone + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Mentha x piperita	-	cv. Black Mitcham	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type enzyme from Escherichia coli strain BL21(DE3) to over 60% homogeneity, and to over 90% purity for the truncated mutant enzyme	Mentha x piperita

Source Tissue

Source Tissue	Comment	Organism	Textmining
epidermis	-	Mentha x piperita	-
leaf	-	Mentha x piperita	-
plant oil gland	-	Mentha x piperita	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(-)-isopiperitenone + NADPH + H+	pathways of monoterpene biosynthesis, overview	Mentha x piperita	(+)-cis-isopulegone + NADP+	-	?
(-)-isopiperitenone + NADPH + H+	regiospecific and stereospecific reductase	Mentha x piperita	(+)-cis-isopulegone + NADP+	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 34409, sequence calculation, 2 * 38000, SDS-PAGE	Mentha x piperita

Synonyms

Synonyms	Comment	Organism
More	(-)-isopiperitenone reductase is a member of the short-chain dehydrogenase/reductase superfamily	Mentha x piperita

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.3	-	NADPH	pH 5.5, recombinant enzyme	Mentha x piperita
1.3	-	(-)-isopiperitenone	pH 5.5, recombinant enzyme	Mentha x piperita

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	wild-type enzyme	Mentha x piperita
6.5	-	truncated mutant enzyme	Mentha x piperita

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	7.5	50% of maximal activity within this range, wild-type enzyme	Mentha x piperita
5	8	50% of maximal activity within this range, deletion mutant enzyme	Mentha x piperita

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	dependent on	Mentha x piperita

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Release 2023.1 (January 2023)