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Literature summary for 1.3.1.9 extracted from

  • Slabas, A.R.; Sidebottom, C.M.; Hellyer, A.; Kessell, R.M.J.; Tombs, M.P.
    Induction, purification and characterization of NADH-specific enoyl acyl carrier protein reductase from developing seeds of oil seed rape (Brassica napus) (1986), Biochim. Biophys. Acta, 877, 271-280.
No PubMed abstract available
Search for more literature for 1.3.1.9

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide no inhibition at 10 mM Brassica napus
N-ethylmaleimide no inhibition at 1 mM, 97% inhibition at 10 mM, inhibition can be partially reversed by dithiothreitol Brassica napus
p-chloromercuribenzoate complete inhibition at 0.1 mM, inhibition can be prevented by preincubation with crotonyl-CoA Brassica napus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 crotonyl-[acyl-carrier protein] Km is 0.001mM or less Brassica napus
0.0076
-
 NADH
-
 Brassica napus
0.178
-
 crotonyl-CoA
-
 Brassica napus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33600
-
 alpha2beta2, 2 *: 34800, 2 * 33600, SDS-PAGE Brassica napus
140000
-
 gel filtration Brassica napus

Organism

Organism UniProt Comment Textmining
Brassica napus
-
 rape
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Brassica napus

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Brassica napus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
200
-
-
 Brassica napus

Storage Stability

Storage Stability Organism
-70°C, at least 3 months, survives only 1 freeze thaw cycle Brassica napus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crotonyl-CoA + NADH
-
 Brassica napus butyryl-CoA + NAD+
-
 ?
crotonyl-[acyl-carrier protein] + NADH
-
 Brassica napus butyryl-[acyl-carrier protein] + NAD+
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha2beta2, 2 *: 34800, 2 * 33600, SDS-PAGE Brassica napus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 in 3-(N-morpholino)propane sulfonate buffer Brassica napus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5
-
 Brassica napus

Cofactor

Cofactor Comment Organism Structure
NADH absolute specificity Brassica napus