Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.9 extracted from

  • Marrakchi, H.; Dewolf, W.E., Jr.; Quinn, C.; West, J.; Polizzi, B.J.; So, C.Y.; Holmes, D.J.; Reed, S.L.; Heath, R.J.; Payne, D.J.; Rock, C.O.; Wallis, N.G.
    Characterization of Streptococcus pneumoniae enoyl-(acyl-carrier protein) reductase (FabK) (2003), Biochem. J., 370, 1055-1062.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 trans-but-2-enoyl-[acyl-carrier protein] 30°C, pH 6.5, in the presence of 100 mM NH4+ Streptococcus pneumoniae
0.023
-
 NADH 30°C, pH 6.5, in the presence of 100 mM NH4+ Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ activation by univalent cations in descending order, NH4+, Rb+, Cs+, K+, and Na+ Streptococcus pneumoniae
K+ activation by univalent cations in descending order, NH4+, Rb+, Cs+, K+, and Na+ Streptococcus pneumoniae
Na+ activation by univalent cations in descending order, NH4+, Rb+, Cs+, K+, and Na+ Streptococcus pneumoniae
NH4+ 15fold activation at 100 mM Streptococcus pneumoniae
Rb+ activation by univalent cations in descending order, NH4+, Rb+, Cs+, K+, and Na+ Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant FabK Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trans-but-2-enoyl-[acyl-carrier protein] + NADH highly selective for NADH Streptococcus pneumoniae butanoyl-[acyl-carrrier-protein] + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
FabK
-
 Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.016
-
 trans-but-2-enoyl-[acyl-carrier protein] 30°C, pH 6.5, in the absence of 100 mM NH4+ Streptococcus pneumoniae
70
-
 trans-but-2-enoyl-[acyl-carrier protein] 30°C, pH 6.5, in the presence of 100 mM NH4+ Streptococcus pneumoniae

Cofactor

Cofactor Comment Organism Structure
FMN
-
 Streptococcus pneumoniae
NADH
-
 Streptococcus pneumoniae