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Literature summary for 1.3.1.9 extracted from
- Mutational analysis of the triclosan-binding region of enoyl-ACP (acyl-carrier protein) reductase from Plasmodium falciparum (2004), Biochem. J., 381, 735-741.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Plasmodium falciparum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A217G | 55% of wild-type kcat | Plasmodium falciparum |
| A217V | 74% of wild-type kcat | Plasmodium falciparum |
| F368A | no activity | Plasmodium falciparum |
| F368I | 65% of wild-type kcat | Plasmodium falciparum |
| M281A | no activity | Plasmodium falciparum |
| M281T | 68% of wild-type kcat | Plasmodium falciparum |
| N218A | no activity | Plasmodium falciparum |
| N218D | 93% of wild-type kcat | Plasmodium falciparum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| triclosan | - |
Plasmodium falciparum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
NADH | 25°C, pH 7.4 | Plasmodium falciparum | |
| 0.16 | - |
trans-but-2-enoyl-[acyl-carrier protein] | 25°C, pH 7.4 | Plasmodium falciparum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38163 | - |
4 * 38163, MALDI-TOF mass spectrometry | Plasmodium falciparum |
| 38166 | - |
4 * 38166, deduced from nucleotide sequence | Plasmodium falciparum |
| 150000 | - |
gel filtration | Plasmodium falciparum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | Q9BH77 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trans-but-2-enoyl-[acyl-carrier protein] + NADH | - |
Plasmodium falciparum | butanoyl-[acyl-carrier protein] + NAD+ | - |
? | |
| trans-but-2-enoyl-[acyl-carrier protein] + NADH + H+ | - |
Plasmodium falciparum | butanoyl-[acyl-carrier protein] + NAD+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 38163, MALDI-TOF mass spectrometry | Plasmodium falciparum |
| tetramer | 4 * 38166, deduced from nucleotide sequence | Plasmodium falciparum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.62 | - |
trans-but-2-enoyl-[acyl-carrier protein] | 25°C, pH 7.4 | Plasmodium falciparum | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Plasmodium falciparum | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000003 | - |
triclosan | 25°C, pH 7.4 | Plasmodium falciparum | |
| 0.00000057 | - |
triclosan | 25°C, pH 7.4, A217G mutant | Plasmodium falciparum | |
| 0.0000015 | - |
triclosan | 25°C, pH 7.4, N218D mutant | Plasmodium falciparum | |
| 0.0000021 | - |
triclosan | 25°C, pH 7.4, N218A mutant | Plasmodium falciparum | |
| 0.0000063 | - |
triclosan | 25°C, pH 7.4, F368I mutant | Plasmodium falciparum | |
| 0.0000072 | - |
triclosan | 25°C, pH 7.4, F368A mutant | Plasmodium falciparum | |
| 0.0000093 | - |
triclosan | 25°C, pH 7.4, M281A mutant | Plasmodium falciparum | |
| 0.00001 | - |
triclosan | 25°C, pH 7.4, M281T mutant | Plasmodium falciparum | |
| 0.00023 | - |
triclosan | 25°C, pH 7.4, A217V mutant | Plasmodium falciparum | |
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