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Literature summary for 1.3.1.9 extracted from

  • Kapoor, N.; Banerjee, T.; Babu, P.; Maity, K.; Surolia, N.; Surolia, A.
    Design, development, synthesis, and docking analysis of 2-substituted triclosan analogs as inhibitors for Plasmodium falciparum enoyl-ACP reductase (2009), IUBMB Life, 61, 1083-1091.
    View publication on PubMed
Search for more literature for 1.3.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His6-tagged ENR in Escherichia coli BL21 Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
2-(2'-amino-4'-chloro-phenoxy)-5-chloro-phenol inhibits the parasite growth, uncompetitive inhibition kinetics with crotonoyl-CoA and competitive with NADH Plasmodium falciparum
4-chloro-1-(4-chloro-2-methoxyphenoxy)-2-nitrobenzene
-
 Plasmodium falciparum
4-[5-chloro-2-(4-chloro-2-hydroxyphenoxy)phenyl]amino-4-oxobutanoic acid
-
 Plasmodium falciparum
4-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]amino-4-oxobutanoic acid
-
 Plasmodium falciparum
5-chloro-2-(4'-chloro-2'-nitro-phenoxy)-phenol inhibits the parasite growth Plasmodium falciparum
5-chloro-2-(4-chloro-2-methoxyphenoxy)aniline
-
 Plasmodium falciparum
5-chloro-2-[4-chloro-2-(prop-1-en-2-ylamino)phenoxy]phenol
-
 Plasmodium falciparum
5-[5-chloro-2-(4-chloro-2-hydroxyphenoxy)phenyl]amino-5-oxopentanoic acid
-
 Plasmodium falciparum
5-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]amino-5-oxopentanoic acid
-
 Plasmodium falciparum
6-[5-chloro-2-(4-chloro-2-hydroxyphenoxy)phenyl]amino-6-oxohexanoic acid
-
 Plasmodium falciparum
6-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]amino-6-oxohexanoic acid
-
 Plasmodium falciparum
additional information structure-based approach for development of 2'-substituted analogues of triclosan as enzyme inhibitors, binding energies and IC50 in culture, overview Plasmodium falciparum
N-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]acetamide
-
 Plasmodium falciparum
triclosan
-
 Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an acyl-[acyl-carrier protein] + NAD+ Plasmodium falciparum
-
 a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged ENR from Escherichia coli BL21 by nickel affinity chromatography Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an acyl-[acyl-carrier protein] + NAD+
-
 Plasmodium falciparum a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?
additional information activity detection via measurement of NADPH production by reduction of crotonoyl-CoA Plasmodium falciparum ?
-
 ?

Synonyms

Synonyms Comment Organism
enoyl-ACP reductase
-
 Plasmodium falciparum
enoyl-acyl carrier protein reductase
-
 Plasmodium falciparum
ENR
-
 Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000096
-
 2-(2'-amino-4'-chloro-phenoxy)-5-chloro-phenol versus NADP+, pH 7.5, 25°C Plasmodium falciparum
0.000104
-
 2-(2'-amino-4'-chloro-phenoxy)-5-chloro-phenol versus crotonoyl-CoA-[acyl-carrier protein], pH 7.5, 25°C Plasmodium falciparum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000073
-
 pH 7.5, 25°C Plasmodium falciparum triclosan
0.00011
-
 pH 7.5, 25°C Plasmodium falciparum 2-(2'-amino-4'-chloro-phenoxy)-5-chloro-phenol
0.000229
-
 pH 7.5, 25°C Plasmodium falciparum 5-chloro-2-(4'-chloro-2'-nitro-phenoxy)-phenol
0.01
-
 pH 7.5, 25°C Plasmodium falciparum 4-chloro-1-(4-chloro-2-methoxyphenoxy)-2-nitrobenzene
0.0122
-
 pH 7.5, 25°C Plasmodium falciparum N-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]acetamide
0.015
-
 pH 7.5, 25°C Plasmodium falciparum 5-chloro-2-(4-chloro-2-methoxyphenoxy)aniline
0.02
-
 pH 7.5, 25°C Plasmodium falciparum 5-chloro-2-[4-chloro-2-(prop-1-en-2-ylamino)phenoxy]phenol
0.0224
-
 pH 7.5, 25°C Plasmodium falciparum 4-[5-chloro-2-(4-chloro-2-hydroxyphenoxy)phenyl]amino-4-oxobutanoic acid
0.0242
-
 pH 7.5, 25°C Plasmodium falciparum 4-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]amino-4-oxobutanoic acid
0.026
-
 pH 7.5, 25°C Plasmodium falciparum 5-[5-chloro-2-(4-chloro-2-hydroxyphenoxy)phenyl]amino-5-oxopentanoic acid
0.0286
-
 pH 7.5, 25°C Plasmodium falciparum 5-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]amino-5-oxopentanoic acid
0.0293
-
 pH 7.5, 25°C Plasmodium falciparum 6-[5-chloro-2-(4-chloro-2-hydroxyphenoxy)phenyl]amino-6-oxohexanoic acid
0.0303
-
 pH 7.5, 25°C Plasmodium falciparum 6-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]amino-6-oxohexanoic acid

General Information

General Information Comment Organism
physiological function PfENR reduces the trans-2 enoyl bond of enoyl-ACP substrates to saturated acyl-ACPs and plays a crucial role in completing the successive rounds of fatty acid elongation Plasmodium falciparum