Literature summary for 1.3.1.91 extracted from

Bou-Nader, C.; Bregeon, D.; Pecqueur, L.; Fontecave, M.; Hamdane, D.
Electrostatic potential in the tRNA binding evolution of dihydrouridine synthases (2018), Biochemistry, 57, 5407-5414 .

Search for more literature for 1.3.1.91

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Saccharomyces cerevisiae
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzyme E294K, vapor diffusion method, using 30% (w/v) PEG 2000 MME, 200 mM ammonium sulfate, and 50 mM sodium acetate pH 5.5. Mutant enzyme E294K/Q305K, vapor diffusion method, using 30% (w/v) PEG 2000 MME, 200 mM ammonium sulfate, and 50 mM sodium acetate pH 5.0. Mutant enzyme Q305K, vapor diffusion method, using 2.2 M ammonium sulfate	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E294K	the mutation increases the tRNA binding affinity and dihydrouridine activity compared to the wild type enzyme	Homo sapiens
E294K/Q305K	the mutation increases the tRNA binding affinity and dihydrouridine activity compared to the wild type enzyme	Homo sapiens
Q305K	the mutation increases the tRNA binding affinity and dihydrouridine activity compared to the wild type enzyme	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
uracil20 in tRNA + NADPH + H+	Saccharomyces cerevisiae	-	5,6-dihydrouracil20 in tRNA + NADP+	-	?
uracil20 in tRNA + NADPH + H+	Homo sapiens	-	5,6-dihydrouracil20 in tRNA + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9NX74	-	-
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex S200 gel filtration	Saccharomyces cerevisiae
Ni-NTA column chromatography and Superdex S200 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
uracil20 in tRNA + NADPH + H+	-	Saccharomyces cerevisiae	5,6-dihydrouracil20 in tRNA + NADP+	-	?
uracil20 in tRNA + NADPH + H+	-	Homo sapiens	5,6-dihydrouracil20 in tRNA + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
dihydrouridine synthase	-	Saccharomyces cerevisiae
dihydrouridine synthase	-	Homo sapiens
DUS2	-	Homo sapiens
Dus2p	-	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
FMN	-	Saccharomyces cerevisiae
FMN	-	Homo sapiens
NADPH	-	Saccharomyces cerevisiae
NADPH	-	Homo sapiens

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Release 2023.1 (January 2023)