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Literature summary for 1.3.1.95 extracted from
- Purification and some properties of an acryloyl-CoA reductase of Clostridium kluyveri (1985), Biol. Chem. Hoppe-Seyler, 366, 953-961.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ethyl acrylate | strong inhibitor of the reduction of ethyl vinyl ketone | Clostridium kluyveri |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00083 | - |
acryloyl-CoA | in 0.1 M phosphate buffer pH 6.4, at 22°C | Clostridium kluyveri | |
| 0.008 | - |
(E)-2-butenoyl-CoA | in 0.1 M phosphate buffer pH 6.4, at 22°C | Clostridium kluyveri | |
| 0.77 | - |
ethyl vinyl ketone | in 0.1 M phosphate buffer pH 6.4, at 22°C | Clostridium kluyveri | |
| 5.6 | - |
acrolein | in 0.1 M phosphate buffer pH 6.4, at 22°C | Clostridium kluyveri | |
| 35 | - |
N-acetyl-S-acryloylcysteamine | in 0.1 M phosphate buffer pH 6.4, at 22°C | Clostridium kluyveri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | does not contain iron | Clostridium kluyveri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14200 | - |
2 * 14200, SDS-PAGE | Clostridium kluyveri |
| 28400 | - |
gel filtration | Clostridium kluyveri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium kluyveri | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| the enzyme exhibits up to 11% and up to 16% residual activity after 19 days of exposure to air at pH 8.0 and 10°C and 30°C, respectively | Clostridium kluyveri |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose CL-6B column chromatography, phenyl-Sepharose CL-4B column chromatography, CM-Sephadex A-25 gel filtration, and Sephacryl S-200 gel filtration | Clostridium kluyveri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, 30% (w/v) ethylene glycol in 15 mM phosphate buffer pH 8.0, 1 year, 20% loss of activity | Clostridium kluyveri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E)-2-butenoate + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
| (E)-2-butenoyl-CoA + methyl viologen | second best substrate, 45% activity compared to acryloyl-CoA | Clostridium kluyveri | ? | - |
? | |
| 1-penten-3-one + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
| 2-cyclohexen-1-one + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
| 2-methylacrolein + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
| 4-phenyl-3-buten-2-one + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
| acrolein + methyl viologen | - |
Clostridium kluyveri | ? | - |
? | |
| acrylic acid + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
| acryloyl-CoA + methyl viologen | best substrate | Clostridium kluyveri | ? | - |
? | |
| butyryl-CoA + 2,6-dichlorophenolindophenol | about 1% of the reduction rate of (E)-2-butenoyl-CoA and methyl viologen | Clostridium kluyveri | ? | - |
? | |
| diethyl ketone + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
| ethyl acrylate + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
| ethyl vinyl ketone + methyl viologen | - |
Clostridium kluyveri | ? | - |
? | |
| methyl propenyl ketone + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
| methyl vinyl ketone + methyl viologen | less than 2% activity of that with ethyl vinyl ketone | Clostridium kluyveri | ? | - |
? | |
| additional information | the enzyme shows no activity with different concentrations of NAD(P)H in 0.1 M phosphate buffer pH 7.0 and ethyl vinyl ketone as substrate | Clostridium kluyveri | ? | - |
? | |
| N-acetyl-S-acryloylcysteamine + methyl viologen | - |
Clostridium kluyveri | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 14200, SDS-PAGE | Clostridium kluyveri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acryloyl-CoA reductase | - |
Clostridium kluyveri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | - |
two optima at pH 5.9 and 6.4 | Clostridium kluyveri |
| 6.4 | - |
two optima at pH 5.9 and 6.4 | Clostridium kluyveri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.9 | 8.3 | the activity of the acryloyl-CoA reductase decreases to about 30% at pH 3.9 or pH 8.3, respectively | Clostridium kluyveri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | contains 1.5 mol FMN | Clostridium kluyveri | |
| additional information | he activity of the acryloyl-CoA reductase is not altered significantly by any of the following substances added in concentrations of 0.075 to 0.15 mM: FMN, FAD, AMP, ADP, ATP, NAD+, NADH, acetyl phosphate and co-enzyme A | Clostridium kluyveri |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Clostridium kluyveri | isoelectric focusing | - |
7.5 |
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