Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FAD | 11-25% stimulation | Enterobacter cloacae |
General Stability | Organism |
---|---|
dithiothreitol stabilizes | Enterobacter cloacae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Enterobacter cloacae | |
N-ethylmaleimide | - |
Enterobacter cloacae | |
p-chloromercuribenzoate | - |
Enterobacter cloacae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine | - |
Enterobacter cloacae | |
0.049 | - |
NADPH | - |
Enterobacter cloacae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | Enterobacter cloacae | - |
UDP-N-acetyl-3-D-muramate + NADP+ | - |
? | |
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | Enterobacter cloacae NRC 492 | - |
UDP-N-acetyl-3-D-muramate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterobacter cloacae | - |
NRC 492 | - |
Enterobacter cloacae NRC 492 | - |
NRC 492 | - |
Oxidation Stability | Organism |
---|---|
very sensitive to oxidation, rapid loss of activity in absence of added dithiothreitol | Enterobacter cloacae |
Purification (Comment) | Organism |
---|---|
- |
Enterobacter cloacae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.015 | - |
- |
Enterobacter cloacae |
Storage Stability | Organism |
---|---|
-20°C, pH 8.0, presence of dithiothreitol, up to 2 months without loss of activity | Enterobacter cloacae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | - |
Enterobacter cloacae | UDP-N-acetyl-3-D-muramate + NADP+ | - |
? | |
UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH | - |
Enterobacter cloacae NRC 492 | UDP-N-acetyl-3-D-muramate + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
increase of activity up to 40°C | Enterobacter cloacae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | 40°C: activity maximum, 50°C: about 30% of activity maximum | Enterobacter cloacae |
50 | - |
at 50°C about 30% of maximal activity | Enterobacter cloacae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | 8.5 | in Tris-HCl buffer maximal activity | Enterobacter cloacae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.1 | 9.9 | at pH 7.1 and pH 9.9 about 60% of maximal activity | Enterobacter cloacae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | prosthetic group: FAD, Na2S2O4, NaBH4 and to a lesser extent NADH can replace NADPH | Enterobacter cloacae | |
additional information | no effect with FMN | Enterobacter cloacae | |
NADH | reductase activity with NADH is only 5% of that with NADPH | Enterobacter cloacae | |
NADPH | - |
Enterobacter cloacae |