Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.1.98 extracted from

  • Gautam, A.; Shukla, H.; Khan, S.; Rishi, P.; Bhakuni, V.; Tewari, R.
    Structural and functional features of UDP-N-acetylenolpyruvylglucosamine reductase of Mycobacterium tuberculosis H37Rv (2010), Int. J. Integr. Biol., 10, 66-75.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ complete inhibition at 10 mM Mycobacterium tuberculosis
guanidine hydrochloride an exponential decrease in enzymatic activity from 100% to 20% is observed between 0 and 0.05 M, no activity is observed above 0.5 M Mycobacterium tuberculosis
Urea no activity at 2.5 M Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0425
-
NADPH pH and temperature not specified in the publication Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required for activation Mycobacterium tuberculosis
additional information not influenced by Mg2+ Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
ESI mass spectrometry Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetylenolpyruvylglucosamine + NADPH + H+
-
Mycobacterium tuberculosis UDP-N-acetylmuramate + NADP+
-
?
UDP-N-acetylenolpyruvylglucosamine + NADPH + H+
-
Mycobacterium tuberculosis H37Rv UDP-N-acetylmuramate + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
EC 1.1.1.158 formerly Mycobacterium tuberculosis
MurB
-
Mycobacterium tuberculosis
UDP-N-acetylenolpyruvylglucosamine reductase
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Mycobacterium tuberculosis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40 at 20 and 40°C more than 85% of the activity is retained Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 between 40°C and 60°C a fast decrease in enzyme activity is observed and above 60°C activity is completely lost Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Mycobacterium tuberculosis

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10 any increase or decrease in pH from 8.0 results in significant drop in functional activity of the enzyme. Complete loss of activity occurs at pH 10.0 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
FAD
-
Mycobacterium tuberculosis