Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | complete inhibition at 10 mM | Mycobacterium tuberculosis | |
guanidine hydrochloride | an exponential decrease in enzymatic activity from 100% to 20% is observed between 0 and 0.05 M, no activity is observed above 0.5 M | Mycobacterium tuberculosis | |
Urea | no activity at 2.5 M | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0425 | - |
NADPH | pH and temperature not specified in the publication | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | required for activation | Mycobacterium tuberculosis | |
additional information | not influenced by Mg2+ | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
ESI mass spectrometry | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylenolpyruvylglucosamine + NADPH + H+ | - |
Mycobacterium tuberculosis | UDP-N-acetylmuramate + NADP+ | - |
? | |
UDP-N-acetylenolpyruvylglucosamine + NADPH + H+ | - |
Mycobacterium tuberculosis H37Rv | UDP-N-acetylmuramate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
EC 1.1.1.158 | formerly | Mycobacterium tuberculosis |
MurB | - |
Mycobacterium tuberculosis |
UDP-N-acetylenolpyruvylglucosamine reductase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Mycobacterium tuberculosis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | at 20 and 40°C more than 85% of the activity is retained | Mycobacterium tuberculosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | between 40°C and 60°C a fast decrease in enzyme activity is observed and above 60°C activity is completely lost | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Mycobacterium tuberculosis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | any increase or decrease in pH from 8.0 results in significant drop in functional activity of the enzyme. Complete loss of activity occurs at pH 10.0 | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Mycobacterium tuberculosis |