Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by DMSO | Escherichia coli | |
NADP+ | about 78% inhibition at 0.06 mM | Escherichia coli | |
thio-NADP+ | about 80% inhibition at 0.06 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
UDP-N-acetylenolpyruvylglucosamine | 10 mM Mg2+, 50 mM Tris-HCl, at pH 8.0, temperature not specified in the publication | Escherichia coli | |
0.0016 | - |
NADPH | in 10 mM PIPES-NaOH (pH 7.0), 3 mM KCl, 2 mM dithiothreitol, 1 mM MgCl, temperature not specified in the publication | Escherichia coli | |
0.0039 | - |
UDP-N-acetylenolpyruvylglucosamine | 10 mM Mg2+, 50 mM Tris-HCl, at pH 8.0 temperature not specified in the publication | Escherichia coli | |
0.0046 | - |
NADPH | 10 mM Mg2+, 50 mM Tris-HCl, at pH 8.0, temperature not specified in the publication | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | K+ activates at 3 mM | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37800 | - |
x * 37800 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Superdex 200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetylenolpyruvylglucosamine + NADPH + H+ | - |
Escherichia coli | UDP-N-acetylmuramate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37800 | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
EC 1.1.1.158 | formerly | Escherichia coli |
MurB | - |
Escherichia coli |
UDP-N-acetylenolpyruvylglucosamine reductase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli |