Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Brassica oleracea var. botrytis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetic acid | - |
Brassica oleracea var. botrytis | |
L-galactono-gamma-lactone | substrate inhibition at concentration above 32.6 mM | Brassica oleracea var. botrytis | |
additional information | no inhibition with riboflavin and lycorine | Brassica oleracea var. botrytis | |
N-ethylmaleimide | - |
Brassica oleracea var. botrytis | |
p-hydroxymercuribenzoic acid | - |
Brassica oleracea var. botrytis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
L-galactono-gamma-lactone | - |
Brassica oleracea var. botrytis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Brassica oleracea var. botrytis | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+, Ca2+ and Sr2+ have no effect on activity | Brassica oleracea var. botrytis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration | Brassica oleracea var. botrytis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + 2 ferricytochrome c | Brassica oleracea var. botrytis | terminal step in L-ascorbic acid biosynthesis | L-ascorbate + 2 ferrocytochrome c + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica oleracea var. botrytis | O47881 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Brassica oleracea var. botrytis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
extract of yeast expressing the recombinant enzyme | Brassica oleracea var. botrytis |
50800 | - |
purified enzyme | Brassica oleracea var. botrytis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + 2 ferricytochrome c | terminal step in L-ascorbic acid biosynthesis | Brassica oleracea var. botrytis | L-ascorbate + 2 ferrocytochrome c + 2 H+ | - |
? | |
L-galactono-1,4-lactone + ferricytochrome c | specific for cytochrome c as electron acceptor | Brassica oleracea var. botrytis | L-ascorbate + ferrocytochrome c | - |
? | |
L-galactono-1,4-lactone + ferricytochrome c | native and recombinant from yeast | Brassica oleracea var. botrytis | L-ascorbate + ferrocytochrome c | - |
? | |
L-galactono-1,4-lactone + ferricytochrome c | specific for L-galactono-gamma-lactone | Brassica oleracea var. botrytis | L-ascorbate + ferrocytochrome c | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 56000, SDS-PAGE | Brassica oleracea var. botrytis |
Synonyms | Comment | Organism |
---|---|---|
GLDase | - |
Brassica oleracea var. botrytis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Brassica oleracea var. botrytis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Brassica oleracea var. botrytis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.8 | - |
Brassica oleracea var. botrytis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | not | Brassica oleracea var. botrytis |