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Literature summary for 1.3.3.11 extracted from

  • Toyama, H.; Chistoserdova, L.; Lidstrom, M.E.
    Sequence analysis of pqq genes required for biosynthesis of pyrroloquinoline quinone in Methylobacterium extorquens AM1 and the purification of a biosynthetic intermediate (1997), Microbiology, 143, 595-602.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
NAD(P)H activating in the presence of EDTA Methylorubrum extorquens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH alpha, complementation studies with PqqC deficient Methylobacterium extorquens and Methylobacterium organophilum Methylorubrum extorquens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41577
-
x * 41577, calculated from the deduced amino acid sequence Methylorubrum extorquens
42000
-
x * 42000, SDS-PAGE, recombinant protein from Escherichia coli Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2 Methylorubrum extorquens synthesis of the pyrroloquinoline quinone (PQQ), that serves as prosthetic group for many bacterial enzymes 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O the product is pyrroloquinoline quinone (PQQ) ?
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2 Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 synthesis of the pyrroloquinoline quinone (PQQ), that serves as prosthetic group for many bacterial enzymes 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O the product is pyrroloquinoline quinone (PQQ) ?

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens Q49150 enzyme appears to be a fusion protein of PqqC and PqqD
-
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 Q49150 enzyme appears to be a fusion protein of PqqC and PqqD
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2
-
Methylorubrum extorquens 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O the product is pyrroloquinoline quinone (PQQ) ?
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2 synthesis of the pyrroloquinoline quinone (PQQ), that serves as prosthetic group for many bacterial enzymes Methylorubrum extorquens 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O the product is pyrroloquinoline quinone (PQQ) ?
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2
-
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O the product is pyrroloquinoline quinone (PQQ) ?
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2 synthesis of the pyrroloquinoline quinone (PQQ), that serves as prosthetic group for many bacterial enzymes Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O the product is pyrroloquinoline quinone (PQQ) ?

Subunits

Subunits Comment Organism
? x * 41577, calculated from the deduced amino acid sequence Methylorubrum extorquens
? x * 42000, SDS-PAGE, recombinant protein from Escherichia coli Methylorubrum extorquens

Synonyms

Synonyms Comment Organism
PqqC
-
Methylorubrum extorquens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Methylorubrum extorquens

pI Value

Organism Comment pI Value Maximum pI Value
Methylorubrum extorquens calculated from the deduced amino acid sequence
-
5.73