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Literature summary for 1.3.3.3 extracted from

  • Goto, T.; Aoki, R.; Minamizaki, K.; Fujita, Y.
    Functional differentiation of two analogous coproporphyrinogen III oxidases for heme and chlorophyll biosynthesis pathways in the cyanobacterium Synechocystis sp. PCC 6803 (2010), Plant Cell Physiol., 51, 650-663.
    View publication on PubMed
Search for more literature for 1.3.3.3

Cloned(Commentary)

Cloned (Comment) Organism
gene sll1185, overexpression of N-terminally Strep-tagged HemF in Escherichia coli Synechocystis sp.

Protein Variants

Protein Variants Comment Organism
additional information construction of enzyme-deficient DELTA sll1185 mutant, which fails to grow under aerobic conditions, with accumulation of coproporphyrin III. This growth defect is restored by cultivation under micro-oxic conditions Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ is added to the assay at 10 mM Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40300
-
 x * 40300, calculated from amino acid sequence Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coproporphyrinogen III + O2 + 2 H+ Synechocystis sp.
-
 protoporphyrinogen IX + 2 CO2 + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 a single hemF gene, sll1185
-
Synechocystis sp. P72848
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant terminally Strep-tagged HemF from Escherichia coli to homogeneity Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrinogen III + O2 + 2 H+
-
 Synechocystis sp. protoporphyrinogen IX + 2 CO2 + 2 H2O
-
 ?
coproporphyrinogen III + O2 + 2 H+ recombinant Sll1185 shows CPO activity under both aerobic and anaerobic conditions Synechocystis sp. protoporphyrinogen IX + 2 CO2 + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 40300, calculated from amino acid sequence Synechocystis sp.

Synonyms

Synonyms Comment Organism
coproporphyrinogen III oxidase
-
 Synechocystis sp.
CPgen oxidase
-
 Synechocystis sp.
CPO
-
 Synechocystis sp.
HemF
-
 Synechocystis sp.
Sll1185
-
 Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Synechocystis sp.

General Information

General Information Comment Organism
metabolism CPO catalyzes the oxidative decarboxylation of coproporphyrinogen III to form protoporphyrinogen IX in heme biosynthesis and is shared in chlorophyll biosynthesis in photosynthetic organisms. HemF plays an essential role under aerobic growth the photosynthetic organism, overview Synechocystis sp.