Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | - |
Saccharomyces cerevisiae | |
dithiothreitol | - |
Saccharomyces cerevisiae | |
glutathione | - |
Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
sensitive towards trypsin and chymotrypsin | Saccharomyces cerevisiae |
stable on dialysis | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenite | slight inhibition | Saccharomyces cerevisiae | |
Co2+ | inhibitory | Saccharomyces cerevisiae | |
Cu2+ | inhibitory | Saccharomyces cerevisiae | |
heme | noncompetitive, reversible by dilution | Saccharomyces cerevisiae | |
hemin | noncompetitive, reversible by dilution | Saccharomyces cerevisiae | |
iodoacetamide | slight inhibition | Saccharomyces cerevisiae | |
iodoacetate | slight inhibition | Saccharomyces cerevisiae | |
additional information | inhibited by high salt concentrations | Saccharomyces cerevisiae | |
p-hydroxymercuribenzoate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
protoporphyrinogen-IX | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen-IX + 3 O2 | Saccharomyces cerevisiae | penultimate step of heme and chlorophyll biosynthesis | protoporphyrin-IX + 3 H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0026 | - |
purified enzyme | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, in 50 mM Tris-HCl in presence of 20% glycerol and 1 mM EDTA, several months, no loss of activity | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mesoporphyrinogen-IX + 3 O2 | 20% of the rate of protoporphyrinogen-IX | Saccharomyces cerevisiae | mesoporphyrin-IX + 3 H2O2 | - |
? | |
protoporphyrinogen-IX + 3 O2 | - |
Saccharomyces cerevisiae | protoporphyrin-IX + 3 H2O2 | - |
? | |
protoporphyrinogen-IX + 3 O2 | penultimate step of heme and chlorophyll biosynthesis | Saccharomyces cerevisiae | protoporphyrin-IX + 3 H2O2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
inactivated above 40°C | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | - |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9.5 | unstable at higher and lower pH | Saccharomyces cerevisiae |