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Literature summary for 1.3.3.4 extracted from

  • Dailey, H.A.; Dailey, T.A.
    Protoporphyrinogen oxidase of Myxococcus xanthus. Expression, purification, and characterization of the cloned enzyme (1996), J. Biol. Chem., 271, 8714-8718.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli His-tag inserted for purification Myxococcus xanthus

Inhibitors

Inhibitors Comment Organism Structure
acifluorfen
-
Myxococcus xanthus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
protoporphyrinogen-IX
-
Myxococcus xanthus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49390
-
calculated from amino acid sequence Myxococcus xanthus
50000
-
SDS-PAGE Myxococcus xanthus
100000
-
gel filtration Myxococcus xanthus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrinogen-IX + 3 O2 Myxococcus xanthus penultimate step of heme and chlorophyll biosynthesis protoporphyrin-IX + 3 H2O2
-
?

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cloned enzyme Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoporphyrinogen-IX + 3 O2
-
Myxococcus xanthus protoporphyrin-IX + 3 H2O2
-
?
protoporphyrinogen-IX + 3 O2 penultimate step of heme and chlorophyll biosynthesis Myxococcus xanthus protoporphyrin-IX + 3 H2O2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 50000, SDS-PAGE, gel filtration Myxococcus xanthus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0867
-
protoporphyrinogen-IX
-
Myxococcus xanthus

Cofactor

Cofactor Comment Organism Structure
FAD one noncovalently bound FAD per dimer Myxococcus xanthus