Cloned (Comment) | Organism |
---|---|
overproduction of mitochondrial isozyme by gene amplification in photomixotrophic cells | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-[2-chloro-4-(trifluoromethyl)phenoxyl]-2-nitrobenzoic acid | transgenic YZI-1S cells show a 150fold greater resistance to the inhibitor compared to wild-type cells, inhibition of mitochondrial and chloroplastic isozymes | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | plastidic isozyme | Nicotiana tabacum | 9507 | - |
membrane | both isozymes | Nicotiana tabacum | 16020 | - |
mitochondrion | mitochondrial isozyme | Nicotiana tabacum | 5739 | - |
additional information | activity in chloroplast of wild-type and transgenic cells is similar for both isozymes, while the transgenic mitochondria show 5.3fold higher activity than those from the wild-type | Nicotiana tabacum | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen-IX + 3 O2 | Nicotiana tabacum | - |
protoporphyrin-IX + 3 H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
cv. Samsun NN, 2 isozymes in chloroplasts and mitochondria | - |
Purification (Comment) | Organism |
---|---|
both isozymes partially from cell culture of wild-type and transgenic cells, by chloroplast and mitochondria fractionation, detergent solubilization, and ultracentrifugation | Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | photomixotrophic cells | Nicotiana tabacum | - |
YZI-1S cell | transgenic cell with 150fold greater resistance to inhibitor S23142 | Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity in chloroplast of wild-type and transgenic cells is similar for both isozymes, while the transgenic mitochondria show 5.3fold higher activity than those from the wild-type | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen-IX + 3 O2 | - |
Nicotiana tabacum | protoporphyrin-IX + 3 H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
protox | - |
Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Nicotiana tabacum |