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Literature summary for 1.3.3.5 extracted from

  • Shimizu, A.; Kwon, J.H.; Sasaki, T.; Satoh, T.; Sakurai, N.; Sakurai, T.; Yamaguchi, S.; Samejima, T.
    Myrothecium verrucaria Bilirubin Oxidase and Its Mutants for Potential Copper Ligands (1999), Biochemistry, 38, 3034-3042.
    View publication on PubMed
Search for more literature for 1.3.3.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Aspergillus oryzae Albifimbria verrucaria
expression of H94V, H134/136V, C457V, C457A and H456/458V mutants in Aspergillus oryzae Albifimbria verrucaria

Protein Variants

Protein Variants Comment Organism
C457A no oxidase activity Albifimbria verrucaria
C457V no oxidase activity Albifimbria verrucaria
H134/136V no oxidase activity Albifimbria verrucaria
H456/458V no oxidase activity Albifimbria verrucaria
H94V no oxidase activity Albifimbria verrucaria
M467G weak oxidase activity Albifimbria verrucaria

Metals/Ions

Metals/Ions Comment Organism Structure
copper multicopper enzyme, contains type 1,2 and 3 copper, authentic and recombinant wild-type enzyme contain 4 copper atoms/protein Albifimbria verrucaria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 1 * 63000, recombinant wild-type enzyme, SDS-PAGE Albifimbria verrucaria
66000
-
 1 * 66000, authentic enzyme, SDS-PAGE Albifimbria verrucaria

Organism

Organism UniProt Comment Textmining
Albifimbria verrucaria
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type enzyme and mutants Albifimbria verrucaria

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.074
-
 M467G mutant enzyme Albifimbria verrucaria
24
-
 recombinant wild-type enzyme expressed in Aspergillus oryzae Albifimbria verrucaria
30
-
 authentic enzyme Albifimbria verrucaria

Subunits

Subunits Comment Organism
monomer 1 * 63000, recombinant wild-type enzyme, SDS-PAGE Albifimbria verrucaria
monomer 1 * 66000, authentic enzyme, SDS-PAGE Albifimbria verrucaria