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Literature summary for 1.3.3.5 extracted from

  • Durand, F.; Gounel, S.; Kjaergaard, C.H.; Solomon, E.I.; Mano, N.
    Bilirubin oxidase from Magnaporthe oryzae: an attractive new enzyme for biotechnological applications (2012), Appl. Microbiol. Biotechnol., 96, 1489-1498.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.3.5

Application

Application Comment Organism
environmental protection the BOD from Magnaporthe oryzae is efficient in decolorizing textile dyes such as Remazol brilliant Blue R, making it useful for environmentally friendly industrial applications Pyricularia oryzae

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of holo-enzyme in Pichia pastoris strain GS115, subcloning in Escherichia coli strain DH5alpha Pyricularia oryzae

General Stability

General Stability Organism
the recombinant enzyme shows high tolerance towards urea Pyricularia oryzae

Inhibitors

Inhibitors Comment Organism Structure
NaCl 50% inhibition at about 10 mM, inactivation at 150 mM with substrate conjugated bilirubin Pyricularia oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ required, 4 ions per enzyme molecule Pyricularia oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 x * 49000, recombinant glycoslated enzyme, SDS-PAGE, x * 63975, mature protein, after N-terminal cleavage, sequence determination Pyricularia oryzae
63975
-
 x * 49000, recombinant glycoslated enzyme, SDS-PAGE, x * 63975, mature protein, after N-terminal cleavage, sequence determination Pyricularia oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 bilirubin + O2 Pyricularia oryzae
-
 2 biliverdin + 2 H2O
-
 ?
2 bilirubin + O2 Pyricularia oryzae ATCC MYA-4617
-
 2 biliverdin + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pyricularia oryzae
-
-
-
Pyricularia oryzae ATCC MYA-4617
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant holo-enzyme from Pichia pastoris strain GS115 to homogeneity by hydrophobic interaction chromatography Pyricularia oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 bilirubin + O2
-
 Pyricularia oryzae 2 biliverdin + 2 H2O
-
 ?
2 bilirubin + O2 substrate conjugated bilirubin Pyricularia oryzae 2 biliverdin + 2 H2O
-
 ?
2 bilirubin + O2
-
 Pyricularia oryzae ATCC MYA-4617 2 biliverdin + 2 H2O
-
 ?
2 bilirubin + O2 substrate conjugated bilirubin Pyricularia oryzae ATCC MYA-4617 2 biliverdin + 2 H2O
-
 ?
2,2'-azino-di-[3-ethylbenzthiazoline-6-sulfonic acid] + O2
-
 Pyricularia oryzae ?
-
 ?
2,2'-azino-di-[3-ethylbenzthiazoline-6-sulfonic acid] + O2
-
 Pyricularia oryzae ATCC MYA-4617 ?
-
 ?
additional information in addition to the oxidation of bilirubin to biliverdin and of biliverdin to a purple pigment, BODs utilize also the more traditional laccase substrates like 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine. The enzyme is efficient in decolorizing textile dyes such as Remazol brilliant Blue R Pyricularia oryzae ?
-
 ?
additional information in addition to the oxidation of bilirubin to biliverdin and of biliverdin to a purple pigment, BODs utilize also the more traditional laccase substrates like 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine. The enzyme is efficient in decolorizing textile dyes such as Remazol brilliant Blue R Pyricularia oryzae ATCC MYA-4617 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 49000, recombinant glycoslated enzyme, SDS-PAGE, x * 63975, mature protein, after N-terminal cleavage, sequence determination Pyricularia oryzae

Synonyms

Synonyms Comment Organism
BOD
-
 Pyricularia oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pyricularia oryzae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 purified recombinant enzyme, half-life for deactivation is more than 300 min Pyricularia oryzae
60
-
 purified recombinant enzyme, half-life for deactivation is about 70 min Pyricularia oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 the activity with laccase substrates 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonate) and syringaldazine is assayed at pH 3.6 and pH 6.2, respectively Pyricularia oryzae
7.2
-
 assay at with bilirubin Pyricularia oryzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1513
-
 bilirubin conjugated bilirubin, pH 7.0, 37°C Pyricularia oryzae