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Literature summary for 1.3.3.6 extracted from
- Higher-plant medium- and short-chain acyl-CoA oxidases: identification, purification and characterization of two novel enzymes of eukaryotic peroxisomal.beta-oxidation (1996), Biochem. J., 320, 607-614.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
dodecanoyl-CoA | MCOX | Zea mays |  |
| 0.006 | - |
hexanoyl-CoA | isozyme SCOX | Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Zea mays | 5777 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
? * 15000, isoform SCOX, labile multimeric structure, SDS-PAGE | Zea mays |
| 60000 | - |
isoform SCOX, gel filtration | Zea mays |
| 62000 | - |
isoform MCOX, gel filtration | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
medium-chain specific isoform, MCOX, and short-chain specific isoform, SCOX | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| both isoforms | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.375 | - |
purified isozyme SCOX | Zea mays |
| 5.3 | - |
highly purified isozyme MCOX | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + O2 | isoform SCOX prefers C4-C8 substrates, isoform MCOX prefers C10-C14 substrates | Zea mays | trans-2,3-dehydroacyl-CoA + H2O2 | - |
? | |
| dodecanoyl-CoA + O2 | - |
Zea mays | trans-dodec-2-enoyl-CoA + H2O2 | - |
r | |
| hexanoyl-CoA + O2 | - |
Zea mays | (2E)-hex-2-enoyl-CoA + H2O2 | - |
? | |
| additional information | structural and functional comparison of isoforms with each other and enzymes from other species, regulatory aspects | Zea mays | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | ? * 15000, isoform SCOX, labile multimeric structure, SDS-PAGE | Zea mays |
| monomer | 1 * 62000, isoform MCOX, SDS-PAGE | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACOX | - |
Zea mays |
| MCOX | - |
Zea mays |
| SCOX | - |
Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | 8.6 | isozyme SCOX | Zea mays |
| 8.6 | - |
isozyme MCOX | Zea mays |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 9.3 | both isoforms | Zea mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein with noncovalently bound FAD | Zea mays | |
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